IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients
IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients
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DOI：
10.1016/j.imlet.2009.10.006
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发表时间：
2010-01-04
影响因子：
4.4
通讯作者：
Min, Jun-Ki
中科院分区：
文献类型：
--
作者：
Heo, Yu-Jung;Joo, Young-Bin;Min, Jun-Ki
产生白细胞介素-17的CD 4（+）T细胞（Th 17细胞）是自身免疫性炎性疾病（包括自身免疫性关节炎）中的主要致病细胞组分。IL-10通过IL-10受体信号促进Foxp 3（+）调节性T细胞的产生。本研究的目的是检查作为抗炎细胞因子的IL-10是否对人Th 17细胞的活化具有抑制作用。免疫组化法检测类风湿性关节炎患者组织中IL-17和IL-10的表达。分离人外周血CD 4（+）T细胞并在各种刺激条件下培养。流式细胞仪检测Th 17细胞和调节性T细胞（Treg）。ELISA和RT-PCR检测细胞因子和转录因子的基因表达。IL-17在类风湿关节炎患者中过表达。在自身免疫性关节炎患者中，IL-10治疗显著降低了体外由Th 17分化条件激活的人CD 4（+）T细胞中IL-17产生细胞和RORc表达细胞的数量。IL-10诱导人CD 4（+）T细胞群中的Foxp 3（+）调节性T细胞。我们的研究结果表明，IL-17在自身免疫性疾病患者中过表达，IL-10抑制IL-17的表达。IL-10可用于治疗自身免疫性疾病。(C)2009 Elsevier B. V.保留所有权利。
Interleukin-17-producing CD4(+) T cells (Th17 cells) are the dominant pathogenic cellular component in autoimmune inflammatory diseases, including autoimmune arthritis. IL-10 promotes the generation of Foxp3(+) regulatory T cells via the IL-10 receptor signal. The objective of this study was to examine whether IL-10, which acts as an anti-inflammatory cytokine, has a suppressive effect on the activation of human Th17 cells. Expression of IL-17 and IL-10 was examined immunohistochemically in tissue obtained from rheumatoid arthritis patients. Human peripheral blood CD4(+) T cells were isolated and cultured under various stimulatory conditions. Th17 cells and regulatory T (Treg) cells were detected by flow cytometry. The gene expression of related cytokines and transcription factors were assessed by ELISA and RT-PCR. IL-17 was overexpressed in rheumatoid arthritis patients. IL-10 treatment significantly decreased the numbers of IL-17-producing and RORc-expressing cells among human CD4(+) T cells that had been activated in vitro by Th17-differentiating conditions in autoimmune arthritis patients. IL-10 induced Foxp3(+) regulatory T cells in the human CD4(+) T cell population. Our results demonstrate that IL-17 is overexpressed in autoimmune disease patients and that IL-10 suppresses IL-17 expression. IL-10 may be useful in the treatment of autoimmune diseases. (C) 2009 Elsevier B.V. All rights reserved.