DOSAGE-DEPENDENT TRANSLATIONAL SUPPRESSION IN YEAST SACCHAROMYCES-CEREVISIAE
DOSAGE-DEPENDENT TRANSLATIONAL SUPPRESSION IN YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/yea.320080702
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发表时间：
1992-07-01
期刊：
影响因子：
2.6
通讯作者：
TERAVANESYAN, MD
中科院分区：
文献类型：
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作者：
CHERNOFF, YO;INGEVECHTOMOV, SG;TERAVANESYAN, MD
SUP35 (SUP2) 野生型基因由于拷贝数增加而过度表达，会诱导类似于该基因突变体表型的全能抑制。检测到 extra-SUP35 对中等甚至低拷贝数的影响。此外，仅包含 SUP35 的 5' 侧翼区域和 N 端 100 bp 蛋白质编码序列的片段的过量使用会导致同种异体抑制。多SUP35基因也与染色体外抑制因子-psi不相容。大概是因为误译率很高。由另一种基因 SUP45 (SUP1) 过量引起的抑制效应要低得多，并且只能在源自高拷贝数质粒的一个构建体中检测到。 extra-SUP35，尤其是 extra-SUP45 诱导的抑制受到细胞环境的影响。讨论了预测基因产物平衡是翻译保真度调节关键的模型。
The overexpression of SUP35 (SUP2) wild-type gene, caused by increase of its copy number, induces an omnipotent suppression similar to the phenotype of mutants for this gene. The effect of extra-SUP35 was detected for moderate or even low copy number. Moreover, overdosage of the fragment including only the 5'-flanking region and N-terminal 100 bp of protein-coding sequence of SUP35 leads to allosuppression. Multi-SUP35 gene was also incompatible with extrachromosomal suppressor factor-psi. presumably because of a high level of mistranslation. The suppressor effect caused by overdosage of another gene, SUP45 (SUP1), is much lower and can be detected only for one construction which is derived from high copy number plasmid. Suppression induced by extra-SUP35 and especially by extra-SUP45 is affected by the cell environment. A model predicting that the balance of gene products is a key for regulation of translational fidelity is discussed.