Unraveling the venom components of an encyrtid endoparasitoid wasp Diversinervus elegans
Unraveling the venom components of an encyrtid endoparasitoid wasp Diversinervus elegans
复制标题
解开秀丽隐杆线虫内寄生蜂的毒液成分
DOI：
10.1016/j.toxicon.2017.06.011
复制
发表时间：
2017-09-15
期刊：
影响因子：
2.8
通讯作者：
Zhu, Jia-Ying
中科院分区：
文献类型：
--
作者：
Liu, Nai-Yong;Wang, Jin-Qiang;Zhu, Jia-Ying
寄生蜂 Diversinervus elegans（膜翅目：Encyrtidae）是球虫科臭名昭著的介壳虫的天敌。含有丰富生物活性分子的毒液是寄生蜂用来调节宿主生理的关键毒力因子。尽管从有限的寄生蜂中积累的毒液成分的知识已经深入了解了它们在寄主与寄生蜂相互作用中的作用，但涉及操纵鳞片生理学的毒素仍然很少有记录。在这里，使用综合转录组学和蛋白质组学方法从线虫中鉴定出总共 48 种假定的毒液蛋白。其中大多数如丝氨酸蛋白酶、酯酶和主要蜂王浆蛋白已在其他几种寄生蜂的毒液中发现。首次揭示了几种毒液蛋白，包括三种功能未知的新蛋白。实时定量 PCR 分析表明，16 个毒液基因表现出雌性偏向的表达，这可能对寄生成功很重要。这些数据丰富了我们对寄生蜂毒液进化和多样性的理解，并且无疑将有助于破译功能性毒液蛋白作为害虫防治的潜在候选者。 (C) 2017 Elsevier Ltd. 保留所有权利。
The encyrtid parasitoid, Diversinervus elegans (Hymenoptera: Encyrtidae), is a natural enemy of the notorious scale pests belonging to the family of Coccidae. Venom containing a rich source of bioactive molecules is a key virulent factor used to regulate host physiology by parasitoids. Although knowledge regarding venom constituents accumulated from limited parasitoids has provided insights into their roles in host-parasitoid interaction, toxins involving in manipulating scale physiology remain sparsely documented. Here, a total number of 48 putative venom proteins were identified from D. elegans using an integrative transcriptomic and proteomic approach. The majority of them such as serine protease, esterase, and major royal jelly protein have been found in venom of other several parasitoid species. Several venom proteins including three novel proteins having unknown function were firstly revealed. Quantitative real time PCR analysis demonstrated that 16 venom genes displayed female-biased expression, which might be important for parasitism success. These data enrich our understanding of parasitoid venom evolution and diversity, and will undoubtedly help deciphering functional venom proteins as potential candidates for pest control. (C) 2017 Elsevier Ltd. All rights reserved.