Tissue engineering of trachea-like cartilage grafts by using chondrocyte macroaggregate: Experimental study in rabbits
Tissue engineering of trachea-like cartilage grafts by using chondrocyte macroaggregate: Experimental study in rabbits
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DOI：
10.1111/j.1525-1594.2007.00474.x
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发表时间：
2007-11-01
影响因子：
2.4
通讯作者：
Mao, Tianqiu
中科院分区：
文献类型：
--
作者：
Wu, Wei;Cheng, Xiaobing;Mao, Tianqiu
气管缺损的治疗和管理仍然是头颈外科的挑战。本研究的目的是探索利用软骨细胞大集合体构建组织工程化气管的新方法，并探讨无支架条件下构建圆柱形气管软骨替代物的可行性。将兔软骨细胞扩增并以高密度接种到培养皿中，以形成机械稳定的软骨细胞大聚集体。一旦通过刮取技术收获软骨细胞大聚集体，将其包裹在硅管周围并皮下植入细胞供体兔中。8周后，收集标本并分析大体外观、组织学、生物化学和生物力学特性。将这些值与天然兔软骨进行比较。结果发现，扩增的软骨细胞可以作为一个连贯的细胞大集合体收获，并可以制成管状移植物。体内植入后，成功再生出具有圆柱形结构的软骨样组织。组织学分析显示，工程气管软骨由均匀分布的陷窝嵌入在富含蛋白多糖的基质中组成; II型胶原在该工程气管软骨中也高度表达。结论：基于软骨细胞大集合体策略，可以成功地重建圆柱形气管软骨。该构建体具有高细胞接种效率、良好的营养灌注和最小的炎症反应的优点，这提供了高效的软骨移植替代物，并且可以用于临床实践中遇到的许多气管软骨损失的情况。
Treatment and management of tracheal defects remain challenges in head and neck surgery. The purposes of this study were to explore a novel strategy to fabricate tissue-engineered trachea by using chondrocyte macroaggregate, and evaluate the feasibility of creating tracheal cartilage equivalents grown in the shape of cylindrical structure without scaffold. Chondrocytes from rabbit cartilage were expanded and seeded into a culture dish at high density to form mechanically stable chondrocyte macroaggregate. Once the chondrocyte macroaggregate was harvested by scrapping technique, it was wrapped around a silicon tube and implanted subcutaneously into the cell donor rabbit. Eight weeks later, specimens were harvested and analyzed for gross appearance, and histological, biochemical, and biomechanical properties. These values were compared with native rabbit cartilage. It was found that expanded chondrocytes could be harvested as a coherent cellular macroaggregate and could be fabricated into a tubelike graft. After in vivo implantation, cartilage-like tissue with cylindrical structure was regenerated successfully. Histological analysis showed engineered trachea cartilage consisted of evenly spaced lacunae embedded in a matrix rich in proteoglycans; type II collagen was also highly expressed in this engineered trachea cartilage. In a conclusion, based on the chondrocyte macroaggregate strategy, tracheal cartilage equivalents with cylindrical shape could be successfully reconstructed. This construct has advantages of high cell-seeding efficiency, good nutritional perfusion, and minimal inflammatory reaction, which provided a highly effective cartilage graft substitute and could be useful in many situations of trachea-cartilage loss encountered in clinical practice.