Separation and purification of pyrroloquinoline quinone from fermentation broth by pretreatment coupled with macroporous resin adsorption
Separation and purification of pyrroloquinoline quinone from fermentation broth by pretreatment coupled with macroporous resin adsorption
复制标题
大孔树脂吸附预处理从发酵液中分离纯化吡咯喹啉醌
DOI：
10.1016/j.seppur.2020.117962
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发表时间：
2021-02-15
影响因子：
8.6
通讯作者：
Yang, Xinwei
中科院分区：
文献类型：
--
作者：
Ke, Chongrong;Ren, Yang;Yang, Xinwei
吡咯喹啉醌 (PQQ) 广泛存在于水果、蔬菜和人类母乳中；它被认为是“长寿维生素”，并被用作功能性食品。在这项研究中，开发了一种从甲基营养细菌的粗发酵液中大规模分离和纯化 PQQ 的两步法。通过离心和过滤完成预处理（流程I），然后应用大孔树脂进行进一步纯化（流程II）。根据静态吸附/解吸测试和描述良好的伪一级动力学模型，选择 XAD-1600 作为最适合 PQQ 分离的树脂。平衡吸附数据很好地拟合了 Langmuir 和 Freundlich 等温线，并进行了动态吸附/解吸测试，以标准化放大的 PQQ 纯化程序的操作参数。最终，经过预处理和大孔树脂处理后，PQQ的回收率达到82.57%，纯度为96.23%，从70 L粗发酵液中得到93.62 g纯度超过99%的PQQ晶体（1.674 g/L PQQ）。因此，我们从发酵液中大规模分离和纯化 PQQ 的策略可能有利于满足食品和制药行业对 PQQ 日益增长的需求。
Pyrroloquinoline quinone (PQQ) is widely found in fruits and vegetables and in human breast milk; it is considered a 'longevity vitamin' and is used as a functional food. In this study, a two-step process for the large-scale separation and purification of PQQ from the crude fermentation broth of a methylotrophic bacterium was developed. Pretreatment was accomplished by centrifugation and filtration (Process I), and then macroporous resins were applied for further purification (Process II). On the basis of static adsorption/desorption tests and the well-described pseudo-first order kinetic model, XAD-1600 was chosen as the most suitable resin for PQQ separation. The equilibrium adsorption data were well fitted to both the Langmuir and Freundlich isotherms, and dynamic adsorption/desorption tests were performed to standardize the operational parameters for a scaled-up PQQ purification procedure. Finally, the recovery yield of PQQ with 96.23% purity after pretreatment and macroporous resin treatment reached 82.57%, and 93.62 g PQQ crystals with over 99% purity were obtained from 70 L of crude fermentation broth (1.674 g/L PQQ). Thus, our strategy for the large-scale separation and purification of PQQ from fermentation broth might be beneficial for meeting the increasing demand for PQQ in the food and pharmaceutical industries.