A233 CHARACTERIZATION OF THE DUODENAL MICROBIOME IN PEDIATRIC CELIAC AND INFLAMMATORY BOWEL DISEASE PATIENTS
A233 CHARACTERIZATION OF THE DUODENAL MICROBIOME IN PEDIATRIC CELIAC AND INFLAMMATORY BOWEL DISEASE PATIENTS
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DOI：
10.1093/jcag/gwab049.232
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发表时间：
2022-02-21
影响因子：
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通讯作者：
Stintzi A
中科院分区：
文献类型：
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作者：
Manoogian J;Butcher J;Li J;Mack DR;Stintzi A
乳糜泻（CE）和炎症性肠病（IBD）都是终身胃肠道（GIT）疾病。CE是一种由麸质摄入引发的自身免疫性疾病，可导致小肠绒毛萎缩、隐窝增生和上皮通透性，而IBD的特征是胃肠道慢性、复发性炎症和溃疡。越来越多的证据表明胃肠道微生物与CE和IBD发病机制有关。研究还表明，CE患者患IBD的风险增加，这表明这些疾病之间可能存在联系。十二指肠在CE和IBD中均可受到影响，但与其他胃肠道区域相比，其研究尚不充分。因此，我们的目的是表征儿童CE、IBD和对照患者（n=76、48和57）的十二指肠微生物组，并假设微生物组成在每个诊断组之间会有所不同。我们使用在安大略省东部儿童医院上腔镜检查时收集的粘膜腔界面吸痰液。从这些样本中提取宏基因组DNA，并通过对16S rRNA基因V6高变区进行测序来鉴定细菌分类群。对照组、CE组和IBD组的十二指肠微生物群α-多样性和β-多样性均无明显差异。然而，我们发现在这三个患者群体中，特定分类群的相对丰度存在一些显著差异。特别是，与非ibd对照相比，发现克罗恩病样本中的小异托孢菌富集。CE患者MLI吸入物中也有较高的拟杆菌、乳杆菌、拟副杆菌和葡萄球菌的趋势。这些结果与先前在CE患者中报道的结果相似。最后，在十二指肠MLI抽吸物中发现的趋势与之前在乳糜泻患者唾液微生物组中发现的结果更一致，而不是在大肠中发现的结果。这项工作提供了对十二指肠微生物组成的独特见解；在CE或IBD中尚未完全表征的GIT区域。CIHRGenome加拿大
Both Celiac disease (CE) and inflammatory bowel disease (IBD) are lifelong gastrointestinal tract (GIT) disorders. CE is an autoimmune disorder triggered by gluten consumption and can result in small intestine villus atrophy, crypt hyperplasia and epithelial permeability, while IBD is characterized by chronic, reoccurring inflammation and ulcers in the GIT. There is increasing evidence linking GIT microbes to both CE and IBD pathogenesis. Studies have also shown that CE patients are at increased risk of developing IBD, suggesting a possible link between these diseases. The duodenum can be affected in both CE and IBD, but it is understudied as compared to other GIT regions. We thus aimed to characterize the duodenal microbiome of pediatric CE, IBD and control patients (n=76, 48 and 57 respectively) and hypothesized that the composition of microbes would vary between each diagnostic group. We used mucosal luminal interface aspirates collected during upper endoscopy at the Children’s Hospital of Eastern Ontario. Metagenomic DNA was extracted from these samples and bacterial taxa was characterized by sequencing the V6 hypervariable region of the 16S rRNA gene. Control, CE, and IBD duodenal microbiotas showed no apparent differences in either α-diversity or β-diversity. However, we identified several significant differences between the relative abundances of specific taxa in these three patient groups. In particular, Atopobium parvulum was found to be enriched in Crohn’s disease samples when compared to non-IBD controls. There was also a trend for higher Bacteroides, Lactobacillus, Parabacteroides and Staphylococcus in CE patient MLI aspirates. These results are similar to what has been previously reported in CE patients. Finally, trends found in the duodenal MLI aspirates are more consistent with results previously found in the salivary microbiome in CD patients as opposed to studies of the large intestine. This work provides unique insight into the microbial composition at the duodenum; a GIT region that has not been fully characterized in CE or IBD. CIHRGenome Canada