Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates
Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates
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DOI：
10.1093/bioinformatics/btm589
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发表时间：
2008-02-01
期刊：
影响因子：
5.8
通讯作者：
Iba, Hideo
中科院分区：
文献类型：
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作者：
Fujita, Shuji;Iba, Hideo
动机：就像转录因子一样，miRNA基因在分化、代谢活化、刺激反应和致癌过程中调节基因表达的全局模式。然而，目前很少有人知道miRNA基因表达本身是如何调控的，因为缺乏其基因结构的基本信息。对miRNA基因启动子区的全局预测将使我们能够探索涉及这些miRNA的基因调控机制的潜在机制。结果：我们推测，如果特定的miRNA分子参与脊椎动物进化保守的调控系统，这将导致miRNA基因启动子的高度保守性以及miRNA分子。在此基础上，通过我们目前对miRNA基因的推定启动子区域（miPPRs）的筛选，我们鉴定了59个miPPRs，它们将指导79个miRNA的产生。我们提出了这些假定的启动子的生物化学和生物信息学验证。
Motivation: Just as transcription factors, miRNA genes modulate global patterns of gene expression during differentiation, metabolic activation, stimulus response and also carcinogenesis. However, little is currently known how the miRNA gene expression itself is regulated owing to lack of basic information of their gene structure. Global prediction of promoter regions of miRNA genes would allow us to explore the mechanisms underlying gene-regulatory mechanisms involving these miRNAs.Results: We speculate that if specific miRNA molecules are involved in evolutionarily conserved regulatory systems in vertebrates, this would entail a high level of conservation of the promoter of miRNA gene as well as the miRNA molecule. By our current screening of putative promoter regions of miRNA genes (miPPRs) on this base, we identified 59 miPPRs that would direct production of 79 miRNAs. We present both biochemical and bioinformatical verifications of these putative promoters.