INVIVO AND INVITRO OBSERVATIONS ON LAMININ PRODUCTION BY SCHWANN-CELLS
INVIVO AND INVITRO OBSERVATIONS ON LAMININ PRODUCTION BY SCHWANN-CELLS
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DOI：
10.1073/pnas.80.12.3850
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
BUNGE, RP
中科院分区：
文献类型：
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作者：
CORNBROOKS, CJ;CAREY, DJ;BUNGE, RP
最近关于营养不良小鼠突变体的报道表明，周围神经组织中突出的细胞外基质成分在周围神经发育中起着重要作用。2个突出的细胞外基质成分，纤连蛋白和层粘连蛋白，无论是在成熟的[大鼠]周围神经在体内和在体外系统，允许在各种功能状态下的雪旺细胞的研究的处置进行了检查。在整个神经的冷冻切片中，用纤连蛋白和层粘连蛋白的抗体染色显示，纤连蛋白染色遍及神经内膜，而层粘连蛋白染色仅限于已知含有基底层的区域，特别是每个成鞘雪旺细胞的基底层。组织培养研究表明，纤连蛋白染色在光学显微镜水平是一个可靠的标志物成纤维细胞（而不是雪旺细胞）在文化;相反，层粘连蛋白的抗体染色成分相关的雪旺细胞表面，但不与成纤维细胞相关的组件。培养的雪旺细胞在发育的所有阶段都产生层粘连蛋白，无论是否与轴突接触。当培养的雪旺细胞开始包裹轴突时，其表面的层粘连蛋白点状区域变得汇合。鞘化完成后，在雪旺细胞基底层区域发现连续的染色线。雪旺细胞只有在与轴突接触时才产生基膜。层粘连蛋白的产生似乎是必要的，但不足以形成基膜。雪旺细胞层粘连蛋白的恒定产生为在组织培养中区分雪旺细胞和成纤维细胞提供了可靠的基础。
Recent reports on the dystrophic mouse mutant suggest that the prominent extracellular matrix component of peripheral nerve tissues plays an important role in peripheral nerve development. The disposition of 2 prominent extracellular matrix components, fibronectin and laminin, both in mature [rat] peripheral nerve in vivo and in an in vitro system that allows study of Schwann cells in various functional states was examined. In frozen sections of whole nerve, staining with antibodies to fibronectin and laminin shows that fibronectin stains throughout the endoneurium while laminin staining is restricted to regions known to contain basal lamina, particularly the basal lamina of each ensheathing Schwann cell. Tissue culture studies indicate that fibronectin staining at the light microscopic level is a reliable marker for fibroblasts (and not Schwann cells) in culture; conversely, antibodies to laminin stain components related to the Schwann cell surface but not components related to fibroblasts. Schwann cells in culture produce laminin at all stages in developed, whether in contact with axons or not. As Schwann cells in culture begin to ensheathe axons, punctate regions of laminin on their surfaces become confluent. After ensheathment is completed, a continuous line of staining is found in the region of the Schwann cell basal lamina. Schwann cells produce a basal lamina only when in contact with axons. The production of laminin appears to be necessary but not sufficient for basal lamina formation. The constancy of laminin production by Schwann cells provides a reliable basis for distinguishing between Schwann cells and fibroblasts in tissue culture.