Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs
Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs
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DOI：
10.1007/s00535-012-0687-8
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发表时间：
2013-05-01
影响因子：
6.3
通讯作者：
Kuwano, Hiroyuki
中科院分区：
文献类型：
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作者：
Yanai, Mitsuhiro;Mochiki, Erito;Kuwano, Hiroyuki
传统的日本医学，被称为汉方医学，由几种草药的混合物组成，广泛用于治疗日本的上消化道疾病。Rikkunshito是这些药物之一，尚未评估其对胃肠道运动活动的影响。我们研究了rikkunshito对清醒狗上消化道运动和血浆ghrelin浓度的影响，并通过手术植入的力传感器研究了胃内注射rikkunshito的收缩反应。在喂食前，将溶解在水中的rikkunshito粉末提取物（1.3、2.7和4.0 g）给予正常和迷走神经切断犬的胃中，并评价胃排空。几种胃肠动力抑制剂（阿托品，六甲铵，昂丹司琼）静脉注射前灌胃给药的rikunshito。在禁食状态下，灌胃给予力健素可引起正常犬十二指肠和空肠的时相性收缩，测定灌胃给予力健素后血浆酰化ghrelin水平。阿托品、六烃季铵和昂丹司琼可抑制Rikkunshito引起的收缩。在切断迷走神经的狗中，rikkunshito诱导了与正常狗相似的阶段性收缩。胃内给予力坤师托以剂量依赖性方式加速胃排空。灌胃给药4.0 g力健素后150 min，血浆酰化ghrelin水平显著高于对照值，胃内给药力健素通过胆碱能神经元和5-HT 3型受体刺激消化间期的胃肠收缩。此外，rikunshito增加血浆酰化ghrelin水平。Rikkunshito可能通过其促动力作用减轻胃肠道疾病。
Traditional Japanese medicine, known as Kampo medicine, consists of mixtures of several medicinal herbs widely used to treat upper gastrointestinal disorders in Japan. Rikkunshito, one of these medicines, has not been evaluated with respect to its influence on gastrointestinal motor activity. We investigated the effect of rikkunshito on upper gastrointestinal motility and plasma ghrelin concentrations in conscious dogs.Contractile response to intragastric administration of rikkunshito was studied via surgically implanted force transducers. A powdered extract of rikkunshito (1.3, 2.7, and 4.0 g) dissolved in water was administered into the stomachs of normal and vagotomized dogs before feeding and gastric emptying was evaluated. Several inhibitors of gastrointestinal motility (atropine, hexamethonium, and ondansetron) were injected intravenously before intragastric administration of rikkunshito. Plasma acylated ghrelin levels after intragastric administration of rikkunshito were measured.In a fasting state, intragastric administration of rikkunshito induced phasic contractions in the duodenum and jejunum in normal dogs. Rikkunshito-induced contractions were inhibited by atropine, hexamethonium and ondansetron. In vagotomized dogs, rikkunshito induced phasic contractions, similar to normal dogs. Gastric emptying was accelerated by intragastric administration of rikkunshito in a dose-dependent manner. The plasma acylated ghrelin level 150 min after intragastric administration of 4.0 g of rikkunshito was significantly higher than the control value.Intragastric administration of rikkunshito stimulates gastrointestinal contractions in the interdigestive state through cholinergic neurons and 5-HT type 3 receptors. Moreover, rikkunshito increases plasma acylated ghrelin levels. Rikkunshito may alleviate gastrointestinal disorders through its prokinetic effects.