The anterior midgut of larval yellow fever mosquitoes (Aedes aegypti): effects of amino acids, dicarboxylic acids, and glucose on the transepithelial voltage and strong luminal alkalinization.
The anterior midgut of larval yellow fever mosquitoes (Aedes aegypti): effects of amino acids, dicarboxylic acids, and glucose on the transepithelial voltage and strong luminal alkalinization.
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黄热病蚊子幼虫（埃及伊蚊）的前中肠：氨基酸、二羧酸和葡萄糖对跨上皮电压和强管腔碱化的影响。
DOI：
10.1002/jez.561
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Onken,Horst
中科院分区：
文献类型：
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作者：
Izeirovski,Sejmir;Moffett,StaciaB;Moffett,DavidF;Onken,Horst
埃及伊蚊幼虫前中肠用含5-羟色胺的充气蚊虫生理盐水(0.2mmoL L−1)浸泡，再用氯化钠灌流(100mmoL L−1)。测量腔内负跨上皮电压(VTE)，通过腔内灌注停止后间甲酚紫色由黄色变为紫色来判断腔内碱化程度。在灌流液中加入10 L−1氨基酸(精氨酸、谷氨酰胺、组氨酸或脯氨酸)或二元酸(苹果酸或琥珀酸)可使VTE值负值增加，而添加葡萄糖则无影响。在以Tris氯化物为灌流液的情况下，营养素的添加不改变Vte。这些结果与氨基酸和二元酸对Na+的吸收是一致的。5-羟色胺的撤除效应表明，这种激素刺激了营养物质的吸收。血淋巴侧含5-羟色胺的蚊虫生理盐水和管腔内的100 L−1NaC l有较强的管腔碱化作用，说明管腔碱化不依赖管腔营养物质。去掉血淋巴侧液中的葡萄糖或二元酸对鲁米那碱化没有影响，而去掉氨基酸则显著减慢鲁米那碱化的速度。氨基酸的重新添加恢复了碱化作用，表明氨基酸代谢参与了鲁米那碱化作用。祖尔。311A：719-726,2009。©2009威利-利斯，Inc.
Isolated anterior midguts of larvalAedes aegyptiwere bathed in aerated mosquito saline containing serotonin (0.2 µmol L−1) and perfused with NaCl (100 mmol L−1). The lumen negative transepithelial voltage (Vte) was measured and luminal alkalinization was determined through the color change of luminal m‐cresol purple from yellow to purple after luminal perfusion stops. Addition of 10 mmol L−1amino acids (arginine, glutamine, histidine or proline) or dicarboxylic acids (malate or succinate) to the luminal perfusate resulted in more negativeVtevalues, whereas addition of glucose was without effect. In the presence of TRIS chloride as luminal perfusate, addition of nutrients did not changeVte. These results are consistent with Na+‐dependent absorption of amino acids and dicarboxylic acids. Effects of serotonin withdrawal indicated that nutrient absorption is stimulated by this hormone. Strong luminal alkalinization was observed with mosquito saline containing serotonin on the hemolymph‐side and 100 mmol L−1NaCl in the lumen, indicating that alkalinization does not depend on luminal nutrients. Omission of glucose or dicarboxylic acids from the hemolymph‐side solution had no effect on luminal alkalinization, whereas omission of amino acids significantly decelerated it. Re‐addition of amino acids restored alkalinization, suggesting the involvement of amino acid metabolism in luminal alkalinization.J. Exp. Zool. 311A: 719–726, 2009. © 2009 Wiley‐Liss, Inc.