POTENTIAL ROLE FOR A FTZ-F1 STEROID-RECEPTOR SUPERFAMILY MEMBER IN THE CONTROL OF DROSOPHILA METAMORPHOSIS
POTENTIAL ROLE FOR A FTZ-F1 STEROID-RECEPTOR SUPERFAMILY MEMBER IN THE CONTROL OF DROSOPHILA METAMORPHOSIS
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DOI：
10.1073/pnas.90.7.3004
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发表时间：
1993-04-01
影响因子：
11.1
通讯作者：
WU, C
中科院分区：
文献类型：
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作者：
LAVORGNA, G;KARIM, FD;WU, C
FTZ-F1是类固醇受体超家族的一员，在果蝇胚胎发生早期与同源盒分割基因fushi tarazu的激活有关。我们克隆了FTZ-F1的发育异构体，发现它是在75CD时作为先前鉴定的蛹前中部染色体膨化的产物表达的。75CD泡芙发生在一段强烈的泡芙活动期间，这是在变态开始时对类固醇激素蜕皮激素的反应所触发的。果蝇多烯染色体上的FTZ-F1的间接免疫荧光染色显示其与超过150个染色体靶点结合，其中包括75CD本身和突出的蛹前膨化，这些膨化被预测由蛹前膨化蛋白调节。这些结果表明，FTZ-F1是昆虫变态的调节因子，并强调了一种调节蛋白在广泛独立的发育途径中的重复利用。
FTZ-F1, a member of the steroid receptor superfamily, has been implicated in the activation of the homeobox segmentation gene fushi tarazu early in Drosophila embryogenesis. We have cloned a developmental isoform of FTZ-F1 and found that it is expressed as a product of the previously identified, midprepupal chromosome puff at 75CD. The 75CD puff occurs in the midst of a period of intense puffing activity that is triggered in response to the steroid hormone ecdysone at the onset of metamorphosis. Indirect immunofluorescent staining for FTZ-F1 on Drosophila polytene chromosomes reveals binding to over 150 chromosomal targets, which include 75CD itself and prominent late prepupal puffs that are predicted to be regulated by midprepupal puff proteins. These results suggest a role for FTZ-F1 as a regulator of insect metamorphosis and underscore the repeated utilization of a regulatory protein for widely separate developmental pathways.