Nested core collections maximizing genetic diversity in Arabidopsis thaliana
Nested core collections maximizing genetic diversity in Arabidopsis thaliana
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DOI：
10.1111/j.1365-313x.2004.02034.x
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发表时间：
2004-04-01
期刊：
影响因子：
7.2
通讯作者：
Brunel, D
中科院分区：
文献类型：
--
作者：
McKhann, HI;Camilleri, C;Brunel, D
要成功地利用自然遗传多样性，就必须对一个物种中存在的变异程度有基本的了解。为了研究拟南芥的自然变异，我们定义了嵌套的核心集合，最大限度地提高了世界范围内的265个加入之间的多样性。核心集合是基于来自均匀分布在基因组中的有限数量的片段的DNA序列数据生成的，并且被证明成功地捕获了其他基因座中的分子多样性以及形态多样性。这些核心集合可供科学界使用，因此为拟南芥遗传变异及其功能后果的研究提供了重要资源。此外，这种策略可以用于其他物种，以提供一个合理的框架进行多样性调查，包括单核苷酸多态性（SNP）的发现和表型，允许利用遗传变异的复杂性状的研究。
The successful exploitation of natural genetic diversity requires a basic knowledge of the extent of the variation present in a species. To study natural variation in Arabidopsis thaliana, we defined nested core collections maximizing the diversity present among a worldwide set of 265 accessions. The core collections were generated based on DNA sequence data from a limited number of fragments evenly distributed in the genome and were shown to successfully capture the molecular diversity in other loci as well as the morphological diversity. The core collections are available to the scientific community and thus provide an important resource for the study of genetic variation and its functional consequences in Arabidopsis. Moreover, this strategy can be used in other species to provide a rational framework for undertaking diversity surveys, including single nucleotide polymorphism (SNP) discovery and phenotyping, allowing the utilization of genetic variation for the study of complex traits.