UNEQUAL MEIOTIC RECOMBINATION WITHIN TANDEM ARRAYS OF YEAST RIBOSOMAL DNA GENES
UNEQUAL MEIOTIC RECOMBINATION WITHIN TANDEM ARRAYS OF YEAST RIBOSOMAL DNA GENES
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DOI：
10.1016/s0092-8674(80)80052-3
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
PETES, TD
中科院分区：
文献类型：
--
作者：
PETES, TD
利用重组DNA程序和酵母转化技术插入酵母基因。2 .编写beta代码。将-苹果酸异丙酯脱氢酶)插入到酵母核糖体DNA基因串联阵列中。遗传和物理技术表明这些插入在减数分裂中是不稳定的。减数分裂不稳定性是位于姐妹染色单体上的核糖体DNA基因簇之间高度不平等重组的结果。
Recombinant DNA procedures and the yeast transformation technique were used to insert the yeast gene .**GRAPHIC**. 2 (coding for .beta.-isopropylmalate dehydrogenase) into the tandem array of ribosomal DNA genes of the yeast Saccharomyces cerevisiae. These insertions were shown by genetic and physical techniques to be unstable in meiosis. The meiotic instability was the result of a high level of unequal recombination between ribosomal DNA gene clusters located on sister chromatids.