Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis
Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis
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hMOF在不同卵巢组织中的表达及其对卵巢癌预后的影响
DOI：
10.3892/or.2014.3649
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发表时间：
2015-02-01
期刊：
影响因子：
4.2
通讯作者：
Lin, Bei
中科院分区：
文献类型：
--
作者：
Cai, Mingbo;Hu, Zhenhua;Lin, Bei
人多器官功能因子(HMOF)是人H4K16的一种主要乙酰化酶，参与生理和病理过程的调节。我们研究了hMOF在不同卵巢组织中的表达及其与卵巢癌预后的关系。逆转录-聚合酶链式反应和蛋白印迹分析分别检测hMOF在不同卵巢组织中的表达。采用免疫组织化学方法检测hMOF在卵巢上皮性癌、交界性肿瘤、良性肿瘤及正常卵巢组织中的表达。此外，还分析了hMOF的表达与卵巢癌临床病理资料的关系。用COX比例风险回归模型分析卵巢癌预后的影响因素。为了分析hMOF表达对卵巢癌预后的影响，对77例卵巢癌患者的随访资料绘制了生存曲线。与正常卵巢组织相比，卵巢上皮性癌组织中hMOF基因和蛋白的表达均显著降低。正常卵巢上皮性肿瘤组织和良性卵巢上皮性肿瘤组织中hMOF高表达的比例明显高于卵巢上皮性癌组织。此外，hMOF蛋白表达与卵巢癌分期密切相关。HMOF蛋白表达是影响卵巢癌预后的独立危险因素。HMOF水平高的患者比hMOF水平低的患者存活率高。HMOF在卵巢上皮性癌中表达降低，hMOF蛋白有可能成为判断卵巢癌预后的新的临床标志物。
Human MOF (hMOF) is a major acetylase of human H4K16 involved in the regulation of physiological and pathological processes. We investigated the expression of hMOF in different ovarian tissues and its correlation with ovarian cancer prognosis. Reverse transcription PCR and western blot analysis were used to detect hMOF mRNA and protein expression, respectively, in different ovarian tissues. Immunohistochemistry was also performed to detect hMOF expression in different ovarian tissues, including ovarian epithelial cancer, borderline tumor, benign tumor and normal ovarian tissues. In addition, the relationships between hMOF expression and clinicopathological ovarian cancer data were analyzed. The Cox proportional-hazards regression model was used to analyze the factors associated with ovarian cancer prognosis. To analyze the effects of hMOF expression on ovarian cancer prognosis, a survival curve was plotted from the follow-up data of 77 patients with ovarian cancer. Compared with normal ovarian tissues, hMOF mRNA and protein expression was significantly decreased in ovarian epithelial cancer tissues. The proportions of high hMOF expression in normal and benign ovarian epithelial tumor tissues, were much higher than those in ovarian epithelial cancer tissues. Furthermore, hMOF protein expression was closely associated with the ovarian cancer stage. The expression of hMOF protein was determined as an independent risk factor influencing ovarian cancer prognosis. Patients with high hMOF levels showed improved survival than those with low hMOF levels. hMOF mRNA and protein expression decreased in ovarian epithelial cancer, thus the hMOF protein potentially serves as a new clinical marker of ovarian cancer prognosis.