Gibberellic Acid-Stimulated Arabidopsis6 Serves as an Integrator of Gibberellin, Abscisic Acid, and Glucose Signaling during Seed Germination in Arabidopsis(1[OPEN])
Gibberellic Acid-Stimulated Arabidopsis6 Serves as an Integrator of Gibberellin, Abscisic Acid, and Glucose Signaling during Seed Germination in Arabidopsis(1[OPEN])
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赤霉酸刺激的拟南芥6 在拟南芥种子萌发过程中充当赤霉素、脱落酸和葡萄糖信号传导的整合者(1[打开])
DOI：
10.1104/pp.15.00858
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发表时间：
2015
期刊：
影响因子：
7.4
通讯作者：
Wang Xiaojing
中科院分区：
文献类型：
--
作者：
Zhong Chunmei;Xu Hao;Ye Siting;Wang Shiyi;Li Lingfei;Zhang Shengchun;Wang Xiaojing
DELLA蛋白RGL2通过多种转录因子在不同条件下对种子萌发起重要作用。然而，与RGL2调控发芽相关的结构基因的功能还不太明确。在这里，我们报告的作用，（拟南芥）细胞壁定位蛋白，赤霉酸刺激的拟南芥6（AtGASA6），在功能上连接RGL2和细胞壁松弛扩展蛋白（拟南芥扩展蛋白A1 [AtEXPA1]），导致胚轴伸长和种子萌发的控制。AtGASA 6过表达的种子表现出早熟萌发，而转移DNA和RNA干扰突变体种子在脱落酸、多效唑和葡萄糖（Glc）胁迫条件下表现出延迟的种子萌发。AtGASA 6基因表达受RGL 2、GLUCOSE INSENSITIVE 2和ABSCISIC ACID-INSENSITIVE 5基因的下调，GASA 6突变体中AtGASA 6表达的缺失逆转了thergl 2突变体对多效唑和thegin 2突变体对葡萄糖诱导的胁迫的不敏感性，这表明它参与调节赤霉素和葡萄糖信号通路。AtGASA 6促进种子萌发和胚轴伸长的作用是通过AtEXPA 1促进胚轴细胞伸长而实现的。综上所述，这些数据表明AtGASA 6 linksRGL 2和AtEXPA 1作为赤霉素、脱落酸和葡萄糖信号传导的整合者发挥功能并发挥作用，从而通过促进细胞伸长来调节种子萌发。
The DELLA protein REPRESSOR OF ga1-3-LIKE2 (RGL2) plays an important role in seed germination under different conditions through a number of transcription factors. However, the functions of the structural genes associated with RGL2-regulated germination are less defined. Here, we report the role of an Arabidopsis (Arabidopsis thaliana) cell wall-localized protein, Gibberellic Acid-Stimulated Arabidopsis6 (AtGASA6), in functionally linking RGL2 and a cell wall loosening expansin protein (Arabidopsis expansin A1 [AtEXPA1]), resulting in the control of embryonic axis elongation and seed germination.AtGASA6-overexpressing seeds showed precocious germination, whereas transfer DNA and RNA interference mutant seeds displayed delayed seed germination under abscisic acid, paclobutrazol, and glucose (Glc) stress conditions. The differences in germination rates resulted from corresponding variation in cell elongation in the hypocotyl-radicle transition region of the embryonic axis.AtGASA6was down-regulated byRGL2,GLUCOSE INSENSITIVE2, andABSCISIC ACID-INSENSITIVE5genes, and loss ofAtGASA6expression in thegasa6mutant reversed the insensitivity shown by thergl2mutant to paclobutrazol and thegin2mutant to Glc-induced stress, suggesting that it is involved in regulating both the gibberellin and Glc signaling pathways. Furthermore, it was found that the promotion of seed germination and length of embryonic axis byAtGASA6resulted from a promotion of cell elongation at the embryonic axis mediated byAtEXPA1. Taken together, the data indicate thatAtGASA6linksRGL2andAtEXPA1functions and plays a role as an integrator of gibberellin, abscisic acid, and Glc signaling, resulting in the regulation of seed germination through a promotion of cell elongation.