Gene expression profiling in acute myeloid leukemia
Gene expression profiling in acute myeloid leukemia
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DOI：
10.1097/01.moh.0000149610.14438.9a
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发表时间：
2005-01-01
影响因子：
3.2
通讯作者：
Löwenberg, B
中科院分区：
文献类型：
--
作者：
Valk, PJM;Delwel, R;Löwenberg, B
本文综述了基因表达谱(GEP)在临床急性髓系白血病(AML)中的应用前景，以及目前对AML新病种的分类和发现所积累的知识。可以推断出具有预测信息的分子签名。监督分析表明，可以用最少的基因集以高精度预测特定的临床相关AML亚集。摘要迄今为止发表的AML GEP研究显示，结果具有显著的一致性，特别是对于类似的GEP平台。这证实了方法学的稳健性和GEP在临床诊断中未来应用的前景。就目前而言，仍有一些技术障碍有待克服。例如，这些问题涉及不同GEP平台之间的数据转换、各种统计聚类方法之间的差异的影响，以及对生物(例如，形态)和遗传因素对表达签名的影响的了解仍然不完全。GEP分析可能与高通量突变分析和蛋白质组学方法相结合，最终可能导致建立一种全面的诊断方法，这将产生AML确切的病理生物学性质的关键。
Purpose of reviewThis review deals with the emerging promises of gene expression profiling (GEP) and the currently accumulating knowledge about the classification and the discovery of novel disease entities in clinical acute myeloid leukemia (AML).Recent findingsGene expression profiling studies in AML have shown that known and novel classes of disease can be recognized by unsupervised analyses. Prognostically informative molecular signatures can be deduced. Supervised analyses show that particular clinically relevant subsets of AML can be predicted with high accuracy with minimal sets of genes.SummaryThe AML GEP studies published to date show a remarkable level of concordance in findings, especially for similar GEP platforms. This confirms the robustness of the methodology and the promise for future applicability of GEP in clinical diagnostics. For the time being, certain technical hurdles remain to be overcome. These relate, for instance, to the conversion of data between different GEP platforms, the effect of differences between various statistical clustering methods, and the still incomplete understanding of the effect of biologic (eg, morphology) and genetic factors on the expression signature. GEP analyses, perhaps in combination with high-throughput mutation analysis and proteomic approaches, may ultimately result in the establishment of a comprehensive diagnostic approach that will yield a key to the precise pathobiologic nature of AML.