Retroviral activation of interleukin 2 gene in a gibbon ape T cell lymphoma line.
Retroviral activation of interleukin 2 gene in a gibbon ape T cell lymphoma line.
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长臂猿猿细胞淋巴瘤线中白介素2基因的逆转录病毒活化。
DOI：
10.1084/jem.164.5.1723
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发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
Kant JA
中科院分区：
文献类型：
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作者：
Durand DB;Kamoun M;Norris CA;Holbrook NJ;Greengard JS;Crabtree GR;Kant JA
长臂猿白血病病毒(GaLVSF)感染的T细胞系MLA 144是从长臂猿淋巴瘤中建立的。该细胞系结构性地表达IL-2及其受体，这意味着自分泌机制可能负责或促进其生长。为了探索IL-2在MLA 144中结构性表达的机制，我们分离并鉴定了代表该细胞系中正常和双插入的IL-2等位基因的粘粒克隆。正常的MLA 144 IL-2等位基因图谱与正常人类IL-2基因的图谱非常相似。该异常等位基因包含一个3‘插入，即带有两个长末端重复序列(LTR)的GaLVSF前病毒和3.25kb的内部缺失。在异常等位基因的5‘端是第二个插入，DNA测序表明是一个分离的GaLVSF LTR，其转录方向与IL-2基因相反。我们通过Northern blotting分析表明，绝大多数转录本来自异常的等位基因，这意味着逆转录病毒的一个或两个插入负责该等位基因的结构性表达。
The gibbon ape leukemia virus (GaLVSF)-infected T cell line, MLA 144, was established from the lymphoma of a gibbon ape. The cell line constitutively expresses IL-2 and its receptor, implying that an autocrine mechanism could be responsible for or contribute toward its growth. To explore the mechanism of constitutive IL-2 expression in MLA 144, we have isolated and characterized cosmid clones representing a normal and a doubly inserted IL-2 allele in this cell line. The map of the normal MLA 144 IL-2 allele closely resembles that of the normal human IL-2 gene. The abnormal allele contains a 3' insertion that is a GaLVSF provirus with two long terminal repeats (LTR) and an internal 3.25 kb deletion. At the 5' end of the abnormal allele is a second insertion that DNA sequencing showed to be an isolated GaLVSF LTR with a transcriptional orientation opposing that of the IL-2 gene. We demonstrate by Northern blotting analysis that the vast majority of transcripts are from the abnormal allele, implying that one or both retroviral insertions are responsible for constitutive expression of the allele.