Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish (Danio rerio)
Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish (Danio rerio)
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DOI：
10.1016/j.aquatox.2014.04.018
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发表时间：
2014-09-01
影响因子：
4.5
通讯作者：
Adam-Guillermin, Christelle
中科院分区：
文献类型：
--
作者：
Bourrachot, Stephanie;Brion, Francois;Adam-Guillermin, Christelle
尽管铀（U）在水环境中的存在特征很好，但人们对鱼类长期暴露于低水平U及其对生殖的潜在影响知之甚少。因此，本研究旨在探讨环境中铀浓度对斑马鱼（Danio rerio）生殖产量及亲鱼暴露后F1代胚胎-仔鱼存活和发育的影响。为此，性成熟的雄性和雌性斑马鱼暴露于20和250微克/升的铀14天，并允许在清洁的水中繁殖，在进一步的14天期间。在所有的采样时间，全身卵黄蛋白原浓度和性腺组织学进行了分析，调查U暴露对这些生殖终点的影响。此外，U在性腺中的积累及其对雄性和雌性性腺细胞的遗传毒性作用进行了量化。结果表明，铀强烈影响鱼类的繁殖能力和产生有活力的个体，表现为产卵量的抑制和F1胚胎死亡率的增加。有趣的是，U暴露导致女性卵黄蛋白原的循环浓度降低。在生殖腺和卵中观察到U浓度增加，这很可能是在母体暴露于U的鱼类生殖腺和胚胎中观察到的遗传毒性效应的原因。总之，这些研究结果突出了环境相关浓度的U的负面影响，改变鱼类的繁殖能力，损害F1胚胎的遗传完整性，进一步关注其在人口水平上的影响。(C)2014爱思唯尔有限公司版权所有。
Despite the well-characterized occurrence of uranium (U) in the aquatic environment, very little is known about the chronic exposure of fish to low levels of U and its potential effect on reproduction. Therefore, this study was undertaken to investigate the effects of environmental concentrations of depleted U on the reproductive output of zebrafish (Danio rerio) and on survival and development of the F1 embryo-larvae following parental exposure to U. For that purpose, sexually mature male and female zebrafish were exposed to 20 and 250 mu g/L of U for 14 days and allowed to reproduce in clean water during a further 14-day period. At all sampling times, whole-body vitellogenin concentrations and gonad histology were analyzed to investigate the effects of U exposure on these reproductive endpoints. In addition, accumulation of U in the gonads and its genotoxic effect on male and female gonad cells were quantified. The results showed that U strongly affected the capability of fish to reproduce and to generate viable individuals as evidenced by the inhibition of egg production and the increased rate of mortality of the F1 embryos. Interestingly, U exposure resulted in decreased circulating concentrations of vitellogenin in females. Increased concentrations of U were observed in gonads and eggs, which were most likely responsible for the genotoxic effects seen in fish gonads and in embryos exposed maternally to U. Altogether, these findings highlight the negative effect of environmentally relevant concentrations of U which alter the reproductive capability of fish and impair the genetic integrity of F1 embryos raising further concern regarding its effect at the population level. (C) 2014 Elsevier B.V. All rights reserved.