Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicale and oligophagous Brevicoryne brassicae
Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicale and oligophagous Brevicoryne brassicae
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DOI：
10.1093/jxb/erm043
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发表时间：
2007-01-01
影响因子：
6.9
通讯作者：
Bones, Atle Magnar
中科院分区：
文献类型：
--
作者：
Kusnierczyk, Anna;Winge, Per;Bones, Atle Magnar
植物具有一系列防御机制来抵御食草昆虫。在十字花科植物中，茉莉酸、水杨酸和乙烯沿着硫代葡萄糖苷及其水解产物在植物保护和植物-昆虫通讯中起着重要作用。反过来，一些食草动物已经适应了含有芥子油苷的植物。作为适应其寄主植物的结果，专业化的昆虫可能会引起不同的植物诱导的反应比通才。寡核苷酸微阵列和qRT-PCR分析被用来表征拟南芥植物的转录谱，以响应感染的通才蚜虫，桃蚜，或十字花科植物专家，短角甘蓝。为了寻找硫苷水解产物不同的植物在分子反应上可能存在的差异和相似之处，研究了三种生态型的A。选择了Thaliana：Wassilewskija（Ws）、Cape Verde Islands（Cvi）和兰茨贝格直立藻（Ler），它们分别主要产生异硫氰酸酯、环硫腈和腈。在所有三个生态型，一般的压力响应基因，属于十八烷和吲哚硫代葡萄糖苷合成途径的基因后，通才和专家的攻击。相比之下，黑芥子酶（水解芥子油苷的酶）的转录受到抑制。茉莉酸合成途径的诱导是最强的Cvi，而上调的吲哚硫代葡萄糖苷合成途径是最高的Ler，这表明在这两个生态型略有不同的防御策略。专家和通才的侵扰造成统计上显着的差异调节60个基因在Ws和21个在Cvi。其中包括茉莉酸和色氨酸合成途径酶，以及致病相关蛋白（PR 1）。昆虫无选择实验表明，降低适合度的B。Brassicae在Ler和Cvi上的繁殖力与Ws相比没有明显的生态类型依赖性变化。桃蚜。在CYP 79 B2和CYP 79 B3基因启动子控制下构建GUS报告基因的靶向研究表明，昆虫特异性诱导吲哚芥子油苷合成途径。
Plants are equipped with a range of defence mechanisms against herbivorous insects. In cruciferous species, jasmonic acid, salicylic acid, and ethylene along with glucosinolates and their hydrolysis products play important roles in plant protection and plant-insect communication. In turn, a number of herbivores have adapted to plants that contain glucosinolates. As a result of adaptation to their host plants, specialized insects may elicit different plant-inducible responses than generalists. Oligonucleotide microarrays and qRT-PCR analysis were used to characterize transcriptional profiles of Arabidopsis thaliana plants in response to infestation with a generalist aphid, Myzus persicae, or a cruciferous plant specialist, Brevicoryne brassicae. To find possible differences and similarities in molecular responses between plants differing in predominant glucosinolate hydrolysis products, three ecotypes of A. thaliana were chosen: Wassilewskija (Ws), Cape Verde Islands (Cvi), and Landsberg erecta (Ler), which, respectively, produce mainly isothiocyanates, epithionitriles, and nitriles. In all three ecotypes, general stress-responsive genes, genes belonging to octadecanoid and indole glucosinolate synthesis pathways were induced upon both generalist and specialist attack. By contrast, transcription of myrosinases, enzymes hydrolysing glucosinolates, was suppressed. The induction of the jasmonic acid synthesis pathway was strongest in Cvi, while the up-regulation of the indole glucosinolate synthesis pathway was highest in Ler, suggesting a slightly different defence strategy in these two ecotypes. Specialist and generalist infestations caused statistically significant differential regulation of 60 genes in Ws and 21 in Cvi. Among these were jasmonic acid and tryptophan synthesis pathway enzymes, and pathogenesis related protein (PR1). Insect no-choice experiments revealed lowered fitness of B. brassicae on Ler and Cvi in comparison to Ws, but no ecotype-dependent change in fecundity of M. persicae. Targeted studies employing constructs of GUS reporter gene under the control of promoters from CYP79B2 and CYP79B3 genes showed insect-specific induction of the indole glucosinolates synthesis pathway.