MOLECULAR ANALYSIS OF THE INITIATION OF INSECT METAMORPHOSIS - A COMPARATIVE-STUDY OF DROSOPHILA ECDYSTEROID-REGULATED TRANSCRIPTION
MOLECULAR ANALYSIS OF THE INITIATION OF INSECT METAMORPHOSIS - A COMPARATIVE-STUDY OF DROSOPHILA ECDYSTEROID-REGULATED TRANSCRIPTION
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DOI：
10.1006/dbio.1993.1315
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发表时间：
1993-12-01
影响因子：
2.7
通讯作者：
THUMMEL, CS
中科院分区：
文献类型：
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作者：
ANDRES, AJ;FLETCHER, JC;THUMMEL, CS
在文献中已经描述了超过50种激素调节的果蝇基因。这些基因是用几种不同的激素反应系统鉴定的，并在各种实验条件下进行了表征。这些方法的多样性使得比较结果和确定对激素的共同反应变得困难。作为表征这些基因通过表皮类固醇的时间调控的第一步，我们使用一组分阶段动物检查了它们在三龄幼虫和蛹前发育过程中的转录活性。在第三龄幼虫发育过程中，我们发现在产卵后78-88小时、100小时、106-108小时和114小时，蜕皮激素调节的基因活性发生了四个坐标变化。在蛹形成时，基因表达发生了一个戏剧性的转变，在此之后，蛹前表皮甾体脉冲诱导了连续的转录波。我们的研究结果表明，在第三龄幼虫和蛹前发育期间，表皮甾体激素滴度的几次增加计划了基因活性的精确时间进展，可以指导变态开始时适当的行为和发育变化。
More than 50 ecdysteroid-regulated Drosophila genes have been described in the literature. These genes were identified using several different ecdysteroid-responsive systems and characterized under a variety of experimental conditions. The diversity of these approaches has made it difficult to compare results and identify common responses to the hormone. As a first step toward characterizing the temporal regulation of these genes by ecdysteroids, we have examined their transcriptional activity throughout third instar larval and prepupal development using a single collection of staged animals. We see four coordinate changes in ecdysteroid-regulated gene activity during third instar larval development, at 78-88 hr, ∼100 hr, 106-108 hr, and ∼114 hr after egg laying. A dramatic transition in gene expression occurs at puparium formation, after which the prepupal ecdysteroid pulse induces successive waves of transcription. Our results suggest that several increases in the ecdysteroid titer during third instar larval and prepupal development program a precise temporal progression of gene activity that could direct the appropriate behavioral and developmental changes at the onset of metamorphosis.