1,6-hexanediol rapidly immobilizes and condenses chromatin in living human cells.
1,6-hexanediol rapidly immobilizes and condenses chromatin in living human cells.
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DOI：
10.26508/lsa.202001005
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发表时间：
2021-04
影响因子：
4.4
通讯作者：
Maeshima K
中科院分区：
文献类型：
--
作者：
Itoh Y;Iida S;Tamura S;Nagashima R;Shiraki K;Goto T;Hibino K;Ide S;Maeshima K
醇，1，6-己二醇，广泛用于熔化液-液相分离形成的液滴;然而，它也可以通过固定和浓缩染色质来严重影响细胞。通过液-液相分离在电池内部形成的液滴保持无膜冷凝物/主体（或隔室）。这些液滴对于浓缩某些分子和促进细胞功能的时空调节是重要的。1，6-己二醇（1，6-HD）是一种脂肪醇，可抑制液滴形成（液滴熔化活性）所需的弱疏水性蛋白质-蛋白质/蛋白质-RNA相互作用，在此用于阐明细胞质/核凝聚物/小体的形成过程。然而，1，6-HD对活细胞中染色质的影响仍不清楚。我们发现，1，6-HD大大抑制染色质运动和超浓缩染色质在人类细胞中，通过使用活细胞单核小体成像，检测染色质状态的变化。这些作用以剂量依赖性方式增强。染色质被5%或更高浓度的1，6-HD“冷冻”。1，6-HD极大地促进了阳离子依赖的染色质体外凝聚。1，6-HD的这种作用不同于其对液滴的熔化活性。酒精，如1，6-HD，似乎可以去除染色质周围的水分子并局部浓缩染色质。因此，当这些液滴与染色质结合时，应仔细解释或重新考虑使用1，6-HD获得的液滴结果。
Alcohol, 1,6-hexanediol, is widely used for melting liquid droplets formed by liquid0–liquid phase separation; however, it can also seriously affect cells by immobilizing and condensing chromatin. Liquid droplets formed inside the cell by liquid–liquid phase separation maintain membrane-less condensates/bodies (or compartments). These droplets are important for concentrating certain molecules and facilitating spatiotemporal regulation of cellular functions. 1,6-hexanediol (1,6-HD), an aliphatic alcohol, inhibits weak hydrophobic protein–protein/protein-RNA interactions required for the droplet formation (droplet melting activity) and is used here to elucidate the formation process of cytoplasmic/nuclear condensates/bodies. However, the effect of 1,6-HD on chromatin in living cells remains unclear. We found that 1,6-HD drastically suppresses chromatin motion and hyper-condenses chromatin in human cells by using live-cell single-nucleosome imaging, which detects changes in the state of chromatin. These effects were enhanced in a dose-dependent manner. Chromatin was “frozen” by 5%, or higher, concentrations of 1,6-HD. 1,6-HD greatly facilitated cation-dependent chromatin condensation in vitro. This 1,6-HD action is distinct from its melting activity of liquid droplets. Alcohols, such as 1,6-HD, appear to remove water molecules around chromatin and locally condense chromatin. Therefore, liquid droplet results obtained using 1,6-HD should be carefully interpreted or reconsidered when these droplets are associated with chromatin.
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影响因子：
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