Establishment of a transgenic zebrafish EF1α:Kaede for monitoring cell proliferation during regeneration
Establishment of a transgenic zebrafish EF1α:Kaede for monitoring cell proliferation during regeneration
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DOI：
10.1016/j.fsi.2013.02.021
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发表时间：
2013-05-01
影响因子：
4.7
通讯作者：
Kim, Cheol-Hee
中科院分区：
文献类型：
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作者：
Moon, Hyun-Yi;Kim, Oc-Hee;Kim, Cheol-Hee
斑马鱼被认为是一种多功能的实验动物，可以用于从发育到疾病的各种研究模型。在这项研究中，我们报道了名为Tg（EF1 α:Kaede）的转基因斑马鱼系的开发，该系表达翻译延伸因子1亚单位α (EF1 α)启动子，该启动子与荧光蛋白（FP） Kaede连接，用于监测再生过程中的增殖细胞。结果表明，EF1 α约1.4 kb的5'侧区足以维持其启动子活性。在成鱼的不同胚胎阶段以及脑、心、胰、肠、卵巢、鱼鳍等器官中均检测到具有绿转红特性的Kaede基因的表达。在切除Tg（EF1 α:Kaede）尾鳍后，对鱼鳍再生过程中的细胞增殖规律进行了监测，结果表明该系统是一种简便、有效的检测组织再生过程中增殖细胞的方法。建立的Tg（EF1 α:Kaede）细胞系具有研究组织损伤后细胞增殖、再生、创面愈合能力和评价创面愈合药物治疗效果的潜力。(C) 2013 Elsevier Ltd.版权所有。
Zebrafish is considered as a versatile experimental animal for various research models from development to diseases. In this study, we report the development of transgenic zebrafish line named as Tg(EF1 alpha:Kaede) that expresses translation elongation factor 1 subunit alpha (EF1 alpha) promoter linked to a fluorescent protein (FP), Kaede for monitoring proliferating cells in during regeneration. It was revealed that about 1.4 kb 5'-flanking region of the EF1 alpha was sufficient for its promoter activity. Expression of Kaede with a property of photo-conversion from green to red was detected in different embryonic stages as well as various organs such as brain, heart, pancreas, intestine, ovary, and fins of adult fish. Cell proliferation pattern during fin regeneration was monitored after amputation of Tg(EF1 alpha:Kaede) caudal fin and results shown that this system is simple and efficient method for detecting proliferating cells during tissue regeneration. Developed Tg(EF1 alpha:Kaede) line has potential to investigate the cell proliferation, regeneration, wound healing capacities after tissue damage and evaluate the therapeutic power of wound healing drugs. (C) 2013 Elsevier Ltd. All rights reserved.