Apoptotic cell death in erythrocytes of p53-deficient medaka (Oryzias latipes) after gamma-irradiation
Apoptotic cell death in erythrocytes of p53-deficient medaka (Oryzias latipes) after gamma-irradiation
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伽马射线照射后 p53 缺陷青鳉 (Oryzias latipes) 红细胞中的细胞凋亡
DOI：
10.1080/09553002.2016.1222091
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Oda S and Mitani H
中科院分区：
文献类型：
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作者：
Sayed AE;Watanabe-Asaka T;Oda S and Mitani H
目的：以往的研究已经研究了γ辐照（γ-IR）对野生型和p53突变体Medaka （Oryzias latipes）照射后24小时的影响，本研究报道了γ射线照射后4、8、24 h和14天红细胞的凋亡和改变作为γ辐照的遗传毒性生物标志物。材料和方法：将性成熟野生型、WT （Hd-rR）和p53（−/−）成年雌性medaka （O. latipes）暴露于4 Gy剂量的γ-IR下，分别于4、8、24 h和14 d后采集样品。结果：野生型小鼠在照射后4 h出现细胞凋亡和形态学改变，照射后8 h细胞凋亡明显增加。在照射后8小时观察到的凋亡细胞死亡在p53突变体medaka中最明显但不明显。照射14天后，两株均恢复了上述表型。虽然在任何组中均未见微核，但在红细胞中观察到核异常。辐照后第24天和第14天红细胞凋亡和形态学改变均明显减少。结论：4gy γ-辐照对鳉鱼红细胞的凋亡和畸形发生在4 - 24 h， 8 h前的初始反应是p53依赖性的。
Purpose:Previous studies have examined the effects of γ-irradiation (γ-IR) on wild-type and p53 mutant Medaka (Oryzias latipes) 24 hours after irradiation and in the present work, apoptosis and alterations in erythrocytes of 4, 8 and 24 h and 14 days after gamma-ray irradiation were reported as genotoxic biomarkers of γ-irradiation.Materials and methods:Sexually mature wild-type, WT (Hd-rR) and p53(−/−) adult female medaka (O. latipes) were exposed to 4 Gy dose of γ-IR and sampling were collected after 4, 8 and 24 h and 14 days.Results:Apoptosis and morphological alterations were observed from 4 h after irradiation and remarkably increased 8 h after irradiation in the wild-type. Apoptotic cell death has been observed 8 h after irradiation most prominently but subtle in p53 mutant medaka. All these phenotypes were recovered 14 days after irradiation in both strains. Although no micronuclei were seen in any group, nuclear abnormalities were observed in red blood cells. Both apoptosis and morphological alterations in erythrocytes were decreased after 24 and 14 days after γ-irradiation.Conclusions:We conclude that apoptosis and malformations caused by 4 Gy γ-irradiation in the erythrocytes of medaka fish occurs from 4–24 h and the initial response until 8 h was p53-dependent.