Initial applications of phospholipid-coated mercury electrodes to the determination of polynuclear aromatic hydrocarbons and other organic micropollutants in aqueous systems
Initial applications of phospholipid-coated mercury electrodes to the determination of polynuclear aromatic hydrocarbons and other organic micropollutants in aqueous systems
复制标题
磷脂涂层汞电极在水系统中多核芳烃和其他有机微污染物测定中的初步应用
DOI：
10.1016/s0003-2670(00)80781-3
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发表时间：
1988
影响因子：
6.2
通讯作者：
J. Readman
中科院分区：
文献类型：
--
作者：
A. Nelson;N. Auffret;J. Readman
研究了双油酰卵磷脂涂层汞电极测定水溶液中多环芳烃(PAHs)和其他微污染物的方法。涂层电极对三环、四环和五环多环芳烃的电容/电压曲线具有良好的响应特性。可逆电容峰的负电位移的程度(从而灵敏度)与PAH上的芳香环和取代基数有关。用相敏交流电记录电容/电压曲线。伏安法。该响应与溶液中多环芳烃的浓度有定量关系。方法检出限为0.4μg L−1，样品重现性为7%。用荧光分光光度法对加标海水样品进行了校正，回收率为80%。该分析系统适用于海水和自来水。牛血清白蛋白(1 mg L−1)增强了对芘的敏感性。该单分子膜对油污水敏感(>；40μg L−1)。电容峰对其他化合物基团和基团中的个别化合物有选择性地响应。单分子膜对多环芳烃的溶液行为似乎高度敏感，因为只有可溶性的未结合的多环芳烃才能穿透单分子膜。添加到溶液中的腐植酸可能通过结合溶液中的一小部分多环芳烃来降低对多环芳烃的反应。
Mercury electrodes coated with di-oleoyl lecithin are described for the determination of polynuclear aromatic hydrocarbons (PAHs) and other micropollutants in aqueous solutions. The coated electrodes give a characteristic response in the capacitance/voltage curve to three-, four- and five- ring PAHs. The extent of the negative potential shift in the reversible capacitance peaks (and thus sensitivity) is related to the number of aromatic rings and substituents on the PAH. The capacitance/voltage curves were recorded by phase-sensitive a.c. voltammetry. The response is quantitatively related to the concentration of PAH in solution. Detection limits are about 0.4 μg l−1and the reproducibility (RSD) for separate samples is 7%. Intercalibration of the method with fluorescence spectrophotometry of pyrene-spiked sea-water samples, showed recoveries of 80%. The analytical system is useful for sea water and tap water. Bovine serum albumin (1 mg l−1) enhances the sensitivity to pyrene. The monolayer is sensitive to oil-contaminated waters ( > 40 μg l−1). The capacitance peaks respond selectively to other groups of compounds and to individual compounds within a group. The monolayer appears highly sensitive to the solution behaviour of PAHs because only soluble unbound PAHs penetrate the monolayer. Humic acid added to the solution decreases the response to PAH presumably by binding a fraction of PAH in the solution.