SEROTYPING OF HUMAN ASTROVIRUS STRAINS BY IMMUNOGOLD STAINING ELECTRON-MICROSCOPY
SEROTYPING OF HUMAN ASTROVIRUS STRAINS BY IMMUNOGOLD STAINING ELECTRON-MICROSCOPY
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DOI：
10.1016/0166-0934(94)90170-8
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发表时间：
1994-12-01
影响因子：
3.1
通讯作者：
KJELDSBERG, E
中科院分区：
文献类型：
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作者：
KJELDSBERG, E
人星状病毒株在CaCo-2细胞培养物中繁殖，并通过免疫吸附电子显微镜（ISEM）证明病毒增殖。采用间接免疫金染色（IGS）电镜技术，以特异性兔抗星状病毒1-6型抗血清为一抗，羊抗兔IgG金标物为二抗，在细胞培养液或粪便提取物中对病毒株进行血清分型。检查了37个星状病毒株，其中26个在细胞培养物中多次传代生长。细胞来源病毒的IGS显示，分别有16、3、3和4株为1、2、3和4型。第5和第6类未被证实。11个菌株在细胞培养物中不生长，并且尝试通过IGS直接在粪便提取物中对这些菌株进行血清分型是不成功的，除了一个菌株被发现是1型。结果表明，IGS可能是一个特定的和合适的方法血清型星状病毒生长在细胞培养物。
Human astrovirus strains were propagated in CaCo-2 cell cultures, and virus multiplication was demonstrated by immunosorbent electron microscopy (ISEM). Serotyping of the virus strains was carried out in cell culture fluids or directly in faecal extracts by an indirect immunogold staining (IGS) electron microscopy technique, using specific rabbit antisera against astrovirus types 1-6 as primary antibodies and goat anti-rabbit IgG gold conjugate as secondary antibody. Thirty-seven astrovirus strains were examined, of which 26 grew in the cell cultures in several passages. IGS of the cell-derived viruses showed that 16, 3, 3, and 4 of the strains were types 1, 2, 3, and 4 respectively. Types 5 and 6 were not demonstrated. Eleven strains did not grow in cell cultures, and attempts to serotype these strains by IGS directly in the faecal extracts were unsuccessful, except for one strain which was found to be type 1. The results indicate that IGS may be a specific and suitable method for serotyping astroviruses grown in cell cultures.