INDUCTION OF THERMOTOLERANCE BY CHEMICAL-AGENTS IN LACTOCOCCUS-LACTIS SUBSP LACTIS IL1403
INDUCTION OF THERMOTOLERANCE BY CHEMICAL-AGENTS IN LACTOCOCCUS-LACTIS SUBSP LACTIS IL1403
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DOI：
10.1016/0168-1605(94)00149-z
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发表时间：
1995-03-01
影响因子：
5.4
通讯作者：
AUFFRAY, Y
中科院分区：
文献类型：
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作者：
BOUTIBONNES, P;BISSON, V;AUFFRAY, Y
与迄今为止测试的其他生物体一样，乳酸乳球菌亚种的适应细胞。在42 ℃下预处理30分钟的乳酸菌IL 1403产生耐热状态，即增加的在随后暴露于致死攻击温度（52 ℃下15或30分钟）下存活的能力。在不同的细胞系统中，二价金属盐、天然或合成化合物等不同的化学物质引发耐热性的发展。然而，在L. lactis subsp.在17种化学物质中，只有氯化镉、氯化汞、叠氮化钠和β-巯基乙醇诱导了这种短暂的耐热性增强，但这四种化合物都没有诱导三种主要热休克蛋白的合成（DnaK、GroEL和hsp 104-analogue），它们被认为是大多数生物体中负责耐热性的。（i）由这些不同的“蛋白毒性”剂产生的损伤与由热产生的损伤根本不同;（ii）热休克蛋白合成和瞬时诱导的耐热性在L. lactis subsp.乳酸菌，因为它们在大肠杆菌和其他一些生物体中。
Like in other organisms tested to date, adapted cells of Lactococcus lactis subsp. lactis IL1403 pretreated at 42 degrees C for 30 min develop a thermotolerant state, i.e. an increased ability to survive subsequent exposure to a lethal challenge temperature (52 degrees C for 15 or 30 min). In different cellular systems, chemicals as diverse as divalent metal salts, natural or synthetic compounds trigger the development of thermotolerance. Yet, in L. lactis subsp. lactis IL1403, among the 17 chemicals tested, only four induced this transient increased tolerance to heat: cadmium chloride, mercury chloride, sodium azide and beta-mercaptoethanol.Intriguingly, none of these four compounds induced the synthesis of three major heat shock proteins (DnaK, GroEL and hsp104-analogue), which are believed to be responsible for thermotolerance in most organisms.It is suggested that: (i) the lesions produced by these various 'proteotoxic' agents are fundamentally different from those produced by heat; (ii) heat shock protein synthesis and transient induced tolerance to heat are not tightly correlated phenomena in L. lactis subsp. lactis as they are in Escherichia coli and some other organisms.