Exploring the protein composition of the plant nuclear envelope
Exploring the protein composition of the plant nuclear envelope
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探索植物核膜的蛋白质组成
DOI：
10.1007/978-1-4939-3530-7_2
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
and I. Meier
中科院分区：
文献类型：
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作者：
X. Zhou;Kentaro Tamura;K. Graumann;and I. Meier
植物核膜和核孔复合体蛋白的靶向鉴定主要有两条途径：（1）先进的计算鉴定和实验验证;（2）复合体的免疫亲和纯化和质谱分析。在候选人鉴定之后，光漂白后荧光恢复（FRAP）和荧光共振能量转移（FRET）提供了强有力的工具来验证NE处的蛋白质-蛋白质相互作用。本文以拟南芥核孔和核膜蛋白的鉴定为例，介绍了这些方法.
Due to rather limited sequence similarity, targeted identification of plant nuclear envelope and nuclear pore complex proteins has mainly followed two routes: (1) advanced computational identification followed by experimental verification and (2) immunoaffinity purification of complexes followed by mass spectrometry. Following candidate identification, fluorescence recovery after photobleaching (FRAP) and fluorescence resonance energy transfer (FRET) provide powerful tools to verify protein–protein interactions in situ at the NE. Here, we describe these methods for the example ofArabidopsis thaliananuclear pore and nuclear envelope protein identification.