Proliferation rate of fibroblast cells on polyethylene surfaces with wettability gradient
Proliferation rate of fibroblast cells on polyethylene surfaces with wettability gradient
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DOI：
10.1002/app.20048
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发表时间：
2004-04-05
影响因子：
3
通讯作者：
Khang, G
中科院分区：
文献类型：
--
作者：
Choee, JH;Lee, SJ;Khang, G
锚定依赖性细胞的表面润湿性对细胞生长速度有重要影响。在我们以前的研究中，我们使用刀式电极的电晕放电处理在聚乙烯(PE)表面制备了润湿性梯度，其功率随着样品长度的增加而逐渐增加。随着功率的增加，PE表面逐渐氧化，并用傅里叶变换红外光谱、接触角测角法和化学分析电子能谱对其进行了表征。本研究的目的是测定不同润湿性的聚合物表面的增殖率。利用电晕处理制备的润湿性梯度PE表面，研究了NIH/3T3成纤维细胞在不同亲水性聚合物表面的生长行为。根据表面润湿性，研究了24至60小时内生长的细胞数量。从PE梯度表面细胞数随培养时间的斜率，计算增殖率(细胞数/cm(2))。H)进行了计算。结果表明，在润湿性梯度表面具有中等亲水性的位置上，细胞的增殖率比在疏水性或亲水性较强的位置上增加得更多，即1111(cell number/cm(2))。H)2.5厘米位置处57度的水接触角(P<；0.05)。这一结果似乎与血清蛋白在表面的吸附密切相关：血清蛋白也更多地吸附在中等亲水性的表面。总之，表面润湿性对细胞在聚合物表面的黏附、铺展和增殖起着重要的作用。(C)2004年威利期刊公司。
Surface wettability on anchorage-dependent cells has an important role in cell growth rate. In our previous studies, we prepared a wettability gradient on polyethylene (PE) surfaces using corona discharge treatment from a knife-type electrode whose power increased gradually along the sample length. The PE surfaces were oxidized gradually with increasing power and characterized by Fourier transform infrared spectroscopy, contact angle goniometry, and electron spectroscopy for chemical analysis. The purpose of this study is to determine the rate of proliferation on polymer surfaces with different wettability. The behavior of cell growth for NIH/3T3 fibroblast cells attached on the polymer surfaces with different hydrophilicity was investigated using wettability gradient PE surfaces prepared by corona discharge treatment. They were investigated for the number of grown cells from 24 to 60 h in terms of surface wettability. From the slope of cell number on PE gradient surface versus culture time, the proliferation rates (number of cell/cm(2) . h) were calculated. It was observed that the proliferation rate was increased more on positions with moderate hydrophilicity of the wettability gradient surface than on the more hydrophobic or hydrophilic positions, i.e., 1111 (number of cell/cm(2) . h) of 57degrees of water contact angle at the 2.5-cm position (P < 0.05). This result seems closely related to the serum protein adsorption on the surface: the serum proteins were also adsorbed more on the moderately hydrophilic surface. In conclusion, surface wettability plays an important role in cell adhesion, spreading, and proliferation on the polymer surfaces. (C) 2004 Wiley Periodicals, Inc.