Characterization of a new β-spectrin gene which is predominantly expressed in brain
Characterization of a new β-spectrin gene which is predominantly expressed in brain
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DOI：
10.1016/s0169-328x(98)00068-0
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发表时间：
1998-06-15
期刊：
影响因子：
--
通讯作者：
Nakayama, M
中科院分区：
文献类型：
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作者：
Ohara, O;Ohara, R;Nakayama, M
我们最近鉴定了一个与β-血影蛋白基因高度相似但与两个已知β-血影蛋白基因中的任一个具有不同染色体位置的基因[T.长濑，K.- I.石川D. Nakajima，M. Ohira，N. Seki，N. Miyajima，A.田中，H. Kotani，N.野村岛大原，未知人类基因编码序列的预测：VII。来自脑的100个新cDNA克隆的完整序列，其可以在体外编码大蛋白质，DNA Res.4（1997）141-150]。为了进一步研究这种新的血影蛋白基因及其产物，我们分离了该基因的大鼠对应物，并分析了其蛋白质切割序列、mRNA及其产物的组织分布以及mRNA及其产物在脑中的区域分布。结果表明，该基因在脑中转录最丰富，神经元是表达该基因的主要细胞类型。特别是，浦肯野细胞中这种基因产物最丰富，这种新形式的β-血影蛋白在树突中比在细胞体中更明显。由于该基因产物的表达模式和亚细胞定位与已经表征的两种β-血影蛋白亚型的表达模式和亚细胞定位完全不同，因此该β-血影蛋白基因将在神经元膜骨架中发挥重要作用，尽管迄今为止它一直被忽视。(C)1998 Elsevier Science B. V.保留所有权利。
We recently identified a gene which shows high similarity to the beta-spectrin gene but with a different chromosomal location from either of the two known beta-spectrin genes [T. Nagase, K.-I. Ishikawa, D. Nakajima, M. Ohira, N. Seki, N. Miyajima, A. Tanaka, H. Kotani, N. Nomura, O. Ohara, Prediction of the coding sequences of unidentified human genes: VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro, DNA Res. 4 (1997) 141-150]. In order to further characterize this new spectrin gene and its product, we isolated the rat counterpart of this gene and analyzed it in terms of its protein ceding sequence, the tissue distribution of its mRNA and the product, and the regional distribution of the mRNA and the product in the brain. The results indicated that this gene was most abundantly transcribed in the brain and neurons were the predominant cell-type to express this gene. In particular, Purkinje cells were the richest in this gene product, and this new form of beta-spectrin was found more prominently in the dendrites than in the cell bodies. Since the expression pattern and the subcellular localization of this gene product were quite distinct from those of the two beta-spectrin isoforms already characterized, this beta-spectrin gene would play an important role in neuronal membrane skeleton although it has been overlooked to date. (C) 1998 Elsevier Science B.V. All rights reserved.