Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals
Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals
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DOI：
10.1016/0304-3940(96)12897-4
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发表时间：
1996-08-23
影响因子：
2.5
通讯作者：
Xin, WJ
中科院分区：
文献类型：
--
作者：
Ni, YC;Zhao, BL;Xin, WJ
在原代培养的大鼠小脑神经元细胞中，研究了氧化应激诱导凋亡的能力以及银杏叶提取物（EGb761）的诱导作用。用20-50 μ M过氧化氢（H2O2）和100 μ M硫酸亚铁处理细胞，使其暴露于羟基自由基。羟基自由基处理以凋亡细胞的典型方式使DNA碎片化，在1%琼脂糖凝胶电泳上产生200个碱基对增量的阶梯模式。100 μ g/ml EGb预处理细胞可减少羟基自由基诱导的细胞凋亡（流式细胞术测定）和DNA断裂。结果表明，羟基自由基可诱导大鼠小脑神经元细胞凋亡，而EGb可抑制这种诱导作用。
The ability of oxidative stress to induce apoptosis, and the effect of Ginkgo biloba extract (EGb761) on this induction were studied in primary cultured rat cerebellar neuronal cells. Cells were exposed to hydroxyl radicals by treating them with 20-50 mu M hydrogen peroxide (H2O2) and 100 mu M ferrous sulfate. Hydroxyl radical treatment fragmented the DNA in a manner typical of apoptosis cells, producing a ladder pattern of 200 base pair increments on 1% agarose gel electrophoresis. Pretreatment of cells with 100 mu g/ml EGb reduced hydroxyl radical induced cells apoptosis (determined by flow cytometry) and DNA fragmentation. The results indicate that hydroxyl radicals induce apoptosis in rat cerebellar neuronal cells, and this induction can be prevented by EGb.