Tyrosine phosphorylation of 100-130 kDa proteins in lung cancer correlates with poor prognosis.
Tyrosine phosphorylation of 100-130 kDa proteins in lung cancer correlates with poor prognosis.
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DOI：
10.1038/bjc.1996.436
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发表时间：
1996-09
影响因子：
8.8
通讯作者：
Hamaguchi, M
中科院分区：
文献类型：
--
作者：
Nishimura, M;Machida, K;Imaizumi, M;Abe, T;Umeda, T;Takeshima, E;Watanabe, T;Ohnishi, Y;Takagi, K;Hamaguchi, M
为了寻找肺癌中与其侵袭行为相关的信号通路，我们研究了肺癌细胞系和手术标本中的酪氨酸磷酸化蛋白。我们发现这些细胞系和不同组织学来源的癌组织的蛋白磷酸化谱密切匹配，并且100-130 kDa蛋白是磷酸化蛋白的主要成分。在手术标本中，大约一半的病例显示这些蛋白以肿瘤特异性的方式酪氨酸磷酸化，并且这些蛋白的磷酸化与术后患者的生存时间有很好的相关性。通过特异性抗体的免疫沉淀，我们发现p125FAK、p120和β -catenin是手术标本中酪氨酸磷酸化蛋白的主要成分。这些结果表明这些蛋白的酪氨酸磷酸化可能在肿瘤复发中起作用，并可作为临床标志物。
To search for the signalling pathways in lung cancer relevant to its aggressive behaviour, we studied tyrosine phosphorylated proteins in lung cancer cell lines and surgical specimens. We found that the profiles of protein phosphorylation were closely matched among these cell lines and cancer tissues of different histological origins, and 100-130 kDa proteins were the major components of phosphorylated proteins. In surgical specimens, approximately half of the cases showed tyrosine phosphorylation of these proteins in a tumour-specific manner, and phosphorylation of these proteins showed good correlation with the survival length of patients after operation. By immunoprecipitation with specific antibodies, we found that p125FAK, p120 and beta-catenin were the major components of tyrosine-phosphorylated proteins in the surgical specimens. These results suggest that tyrosine phosphorylation of these proteins may play a role in tumour relapse and is available as a clinical marker.