PIL5, a phytochrome-interacting basic helix-loop-helix protein, is a key negative regulator of seed germination in Arabidopsis thaliana
PIL5, a phytochrome-interacting basic helix-loop-helix protein, is a key negative regulator of seed germination in Arabidopsis thaliana
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DOI：
10.1105/tpc.104.025163
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发表时间：
2004-11-01
期刊：
影响因子：
11.6
通讯作者：
Choi, G
中科院分区：
文献类型：
--
作者：
Oh, E;Kim, J;Choi, G
被子植物种子发芽与否的第一个决定是基于各种环境信号的整合，如水和光。光敏色素（Phys）作为红光和远红光（Pfr）光受体，通过尚未表征的途径介导光信号。我们在这里报告的PIF 3样5（PIL-5）蛋白，一个基本的螺旋-环-螺旋转录因子，是光敏色素介导的种子萌发的关键负调节。PIL 5优先与光敏色素A（PhyA）和光敏色素B（PhyB）的Pfr形式相互作用。pil 5突变体与phyA和phyB突变体、pif 3 pif 5双突变体和PIL 5过表达系的结合分析表明，PIL 5在Phy介导的促进种子萌发、抑制下胚轴负向地性和抑制下胚轴伸长中是负因子。我们的数据确定PIL 5作为第一个Phy相互作用的蛋白质，调节种子萌发。
The first decision made by an angiosperm seed, whether to germinate or not, is based on integration of various environmental signals such as water and light. The phytochromes (Phys) act as red and far-red light (Pfr) photoreceptors to mediate light signaling through yet uncharacterized pathways. We report here that the PIF3-like 5 (PIL-5) protein, a basic helix-loop-helix transcription factor, is a key negative regulator of phytochrome-mediated seed germination. PIL5 preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB). Analyses of a pil5 mutant in conjunction with phyA and phyB mutants, a pif3 pif5 double mutant, and PIL5 overexpression lines indicate that PIL5 is a negative factor in Phy-mediated promotion of seed germination, inhibition of hypocotyl negative gravitropism, and inhibition of hypocotyl elongation. Our data identify PIL5 as the first Phy-interacting protein that regulates seed germination.