BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells
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DOI：
10.1038/s41598-020-67169-1
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发表时间：
2020-07-09
影响因子：
4.6
通讯作者：
Alarcon, Emilio, I
中科院分区：
文献类型：
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作者：
Cortes, David;McTiernan, Christopher D.;Alarcon, Emilio, I
3D打印用于开发一种开放获取装置，该装置能够同时电刺激和机械刺激6孔板中的人诱导多能干细胞。该设备使用计算机辅助设计（CAD）设计，并使用FDA批准的耐高压灭菌材料进行3D打印。该设备的紧凑设计和材料选择允许其在高湿度下工作的细胞培养箱中使用，而不会有过热或腐蚀的风险。细胞的机械刺激通过柔性半透明硅树脂膜的循环偏转进行，所述柔性半透明硅树脂膜通过真空控制的开放式装置进行。对节律性刺激周期进行编程，以创建生理学上更相关的体外模型。这种机械刺激与原位电刺激耦合并同步。我们评估了我们的设备支持来自人诱导多能干细胞的心肌细胞的能力，证实了在机电刺激下培养的细胞呈现出确定的/成熟的心肌细胞表型。这种3D打印设备提供了一种独特的高通量体外系统，该系统结合了机械和电刺激，因此，我们预计它将在任何电响应组织（如肌肉和神经）的研究中找到应用。
3D printing was used to develop an open access device capable of simultaneous electrical and mechanical stimulation of human induced pluripotent stem cells in 6-well plates. The device was designed using Computer-Aided Design (CAD) and 3D printed with autoclavable, FDA-approved materials. The compact design of the device and materials selection allows for its use inside cell incubators working at high humidity without the risk of overheating or corrosion. Mechanical stimulation of cells was carried out through the cyclic deflection of flexible, translucent silicone membranes by means of a vacuum-controlled, open-access device. A rhythmic stimulation cycle was programmed to create a more physiologically relevant in vitro model. This mechanical stimulation was coupled and synchronized with in situ electrical stimuli. We assessed the capabilities of our device to support cardiac myocytes derived from human induced pluripotent stem cells, confirming that cells cultured under electromechanical stimulation presented a defined/mature cardiomyocyte phenotype. This 3D printed device provides a unique high-throughput in vitro system that combines both mechanical and electrical stimulation, and as such, we foresee it finding applications in the study of any electrically responsive tissue such as muscles and nerves.