Observations on the embolism and survival of Pinus thunbergii seedlings inoculated with Bursaphelenchus xylophilus
Observations on the embolism and survival of Pinus thunbergii seedlings inoculated with Bursaphelenchus xylophilus
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接种松材线虫的黑松幼苗栓塞及成活观察
DOI：
10.1007/s10658-015-0829-y
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发表时间：
2016
影响因子：
1.8
通讯作者：
Endo R
中科院分区：
文献类型：
--
作者：
Umebayashi T;Yamada T;Fukuhara K;Endo R
为了阐明松材线虫（Bursaphelenchusxylophilus）侵染后幼苗存活与木质部栓塞分布之间的关系，我们用酸性品红染料溶液检测了5组3年生黑松幼苗的木质部栓塞面积，其中包括一个敏感组和4个不同程度的抗性组。我们证实，通过适当的程序，可以使用染料摄取法准确描绘PWN感染引起的栓塞区域。在所有组中，接种后45天（DAI），在接种伤口上方或下方1 cm处可见额外栓塞。在所有染色的幼苗，栓塞的分布与轴向树脂道，而不是射线组织。耐药组之间的存活率的差异取决于当年木质部栓塞的分布。45日龄导水面积损失率较高的易感组和两个具有不同程度抗性的组，接种后5个月存活率较低。在许多存活的幼苗中，一般在较新的管胞中观察到水分运动，而栓塞在三组的一些幼苗中延伸到形成层附近。新的管胞的水传导途径的保留被认为是必要的生存感染PWN后。
To elucidate the relationship between seedling survival and the distribution of xylem embolisms after infection with pinewood nematode (PWN),Bursaphelenchus xylophilus, we examined embolised xylem areas using acid fuchsin dye solution in five groups of 3-year-oldPinus thunbergiiseedlings which included a susceptible group and four groups with varying degrees of resistance. We confirmed that embolised areas caused by infection with PWN could be delineated accurately using a dye uptake method with appropriate procedures. In all groups, the additional embolisms were visible 1 cm above or below the inoculation wound 45 days after inoculation (DAI). In all stained seedlings, the distribution of embolisms was associated with axial resin canals and not with ray tissues. The difference in survival rates among the resistant groups depended on the distribution of embolisms in the current-year xylem. A susceptible group and two groups with varying degrees of resistance that showed higher percentage loss of water-conducting area at 45 DAI exhibited low survival rate at 5 months after inoculation. In many surviving seedlings, water movement was generally observed in newer tracheids, while embolisms extended to near the cambium in some seedlings of the three groups. Retention of the water-conducting pathway of newer tracheids is thought to be necessary for survival after infection with PWN.