A simple method for constructing E1-and E1/E4-deleted recombinant adenoviral vectors

A simple method for constructing E1-and E1/E4-deleted recombinant adenoviral vectors
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DOI:
10.1089/10430349950017374
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发表时间:
1999-08-10
期刊:
影响因子:
4.2
通讯作者:
Kay, MA
Kay, MA
中科院分区:
医学2区
文献类型:
--
作者:
Mizuguchi, H;Kay, MA

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我们之前开发了一种双质粒体外连接方法,不需要重组步骤就可以产生新的重组E1或E1/ e3缺失的腺病毒载体。在这项研究中,我们修改了系统,以提高向量构建的简单性,此外,允许产生E1/ e4删除的向量。
We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.