Small RNA deep sequencing reveals the important role of microRNAs in the halophyte Halostachys caspica.
Small RNA deep sequencing reveals the important role of microRNAs in the halophyte Halostachys caspica.
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DOI：
10.1111/pbi.12337
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发表时间：
2015-04
影响因子：
13.8
通讯作者：
Ruirui Yang;You-ling Zeng;Xiaoyan Yi;Lijuan Zhao;Yufang Zhang
中科院分区：
文献类型：
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作者：
Ruirui Yang;You-ling Zeng;Xiaoyan Yi;Lijuan Zhao;Yufang Zhang
MicroRNAs（miRNAs）是一类广泛存在的小分子调控RNA，在植物生长发育和逆境应答中发挥着重要作用。然而，miRNA介导的植物非生物胁迫响应的调控机制尚不清楚。盐穗木是一种盐生植物，是研究植物对盐胁迫响应的理想模式植物。然而，目前还没有关于H.卡斯皮卡在这项研究中，我们采用深度测序来鉴定暴露于盐度的H. caspica及其未处理的对照。在两种处理产生的1300 - 1900万个序列中，共鉴定出170个保守的miRNA，属于151个miRNA家族;其中，31个miRNA在盐胁迫下显着上调，48个显着下调。我们还从H.其中12个miRNAs受盐度影响显著上调，13个显著下调。qRT-PCR表达分析验证了深度测序结果，同时也证明了miRNAs及其靶基因对高盐胁迫的响应，且miRNAs与靶基因之间存在负相关性。miRNA靶点预测、GO和KEGG分析表明，miRNA参与了盐胁迫相关的生物学途径，包括钙信号途径、MAPK信号途径、植物激素信号转导和类黄酮生物合成等，表明miRNA在H.卡斯皮卡这一结果可用于提高作物和林木的耐盐性。
MicroRNAs (miRNAs), an extensive class of small regulatory RNAs, play versatile roles in plant growth and development as well as stress responses. However, the regulatory mechanism is unclear on miRNA-mediated response to abiotic stress in plants. Halostachys caspica is a halophytic plant species and a great model for investigating plant response to salinity stress. However, no research has been performed on miRNAs in H. caspica. In this study, we employed deep sequencing to identify both conserved and novel miRNAs from salinity-exposed H. caspica and its untreated control. Among the 13-19 million sequences generated from both treatments, a total of 170 conserved miRNAs, belonging to 151 miRNA families, were identified; among these miRNAs, 31 were significantly up-regulated and 48 were significantly down-regulated by salinity stress. We also identified 102 novel miRNAs from H. caspica; among them, 12 miRNAs were significantly up-regulated and 13 were significantly down-regulated by salinity. qRT-PCR expression analysis validated the deep sequencing results and also demonstrated that miRNAs and their targeted genes were responsive to high salt stress and existed a negative expression correlation between miRNAs and their targets. miRNA-target prediction, GO and KEGG analysis showed that miRNAs were involved in salt stress-related biological pathway, including calcium signalling pathway, MAPK signalling pathway, plant hormone signal transduction and flavonoid biosynthesis, etc. This suggests that miRNAs play an important role in plant salt stress tolerance in H. caspica. This result could be used to improve salt tolerance in crops and woods.