Mesoporous silica nanoparticles/hydroxyapatite composite coated implants to locally inhibit osteoclastic activity.
Mesoporous silica nanoparticles/hydroxyapatite composite coated implants to locally inhibit osteoclastic activity.
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DOI：
10.1021/am405013t
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发表时间：
2014-04
影响因子：
9.5
通讯作者：
Min Zhu;Yufang Zhu;B. Ni;Ning Xie;Xuhua Lu;Jianlin Shi;Yi Zeng;Xiang Guo
中科院分区：
文献类型：
--
作者：
Min Zhu;Yufang Zhu;B. Ni;Ning Xie;Xuhua Lu;Jianlin Shi;Yi Zeng;Xiang Guo
为了从抗骨吸收的角度改善种植体-骨结合并加速骨折愈合，我们采用了一种新的方法在不锈钢克氏针基体上制备了介孔二氧化硅纳米颗粒/羟基磷灰石（MSNs/HA）复合涂层。表面微观结构的表征表明，与作为对照的HA涂层丝相比，比表面积增大，因此MSN/HA复合材料涂层植入物具有局部递送生物医学物质并促进骨折愈合的能力。在此，唑来膦酸（ZOL）作为一个模型药物，不同剂量的固定在介孔涂层对降低骨吸收活性。ZOL的负载能力增加了近8倍，纯HA涂层，和MSNs的引入明显延缓ZOL释放，实现更持久的释放曲线。破骨细胞样细胞与含ZOL的钢丝共培养一定时间后，进行多核细胞的抗酒石酸酸性磷酸酶（TRAP）染色和陷窝形成试验，以研究ZOL剂量依赖性的抗骨吸收活性。促进破骨细胞的局部作用将具有支持种植体整合和骨修复的临床益处。
In an attempt to improve implant-bone integration and accelerate bone fracture healing from resisting osteoclastic resorption point of view, we have employed a novel procedure to develop a mesoporous silica nanoparticles/hydroxyapatite (MSNs/HA) composite coating onto stainless Kirschner wire substrate. Characterizations of the surface microstructures indicated enlarged specific surface area compared to HA-coated wires as control, thus the MSNs/HA composite coated implants are endowed with abilities to locally deliver biomedical substances and enhance fracture healing. Herein, zoledronic acid (ZOL) as a model drug, different doses of which were immobilized in the mesoporous coating toward decreasing osteoclastic resorption activity. The loading capacities of ZOL increased almost eight-folds to that of pure HA coating, and the introduction of MSNs obviously retarded ZOL release to achieve a more sustained release profile. After certain periods of osteoclast like cells co-culturing with ZOL contained wires, tartrat-resistant acid phosphatases (TRAP) staining of polynucleated cells and a pit formation assay were performed to investigate the ZOL dose-dependent anti-resorption activity. The promoted local effect on osteoclasts will be of clinical benefit to support implant integration and bone repair.