Fertilized eggs obtained from transplantation of frozen ovaries and parthenogenesis in combination with artificial insemination of frozen semen of the silkworm, Bombyx mori
Fertilized eggs obtained from transplantation of frozen ovaries and parthenogenesis in combination with artificial insemination of frozen semen of the silkworm, Bombyx mori
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DOI：
10.1016/s0011-2240(03)00021-x
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发表时间：
2003-04-01
期刊：
影响因子：
2.7
通讯作者：
Horie, Y
中科院分区：
文献类型：
--
作者：
Mochida, YJ;Takemura, Y;Horie, Y
本文报道了一种家蚕卵巢和精子长期保存的可靠方法。提出了三项研究。在第一种方法中，卵巢从第3、4或5龄的幼虫中取出，冷冻保存，并储存在液氮中。将解冻的卵巢移植到相同或不同发育阶段的手术去势雌性幼虫中。产卵的受体雌虫中，以3龄和4龄幼虫的比例最高（分别为22.1%和8.7%）。同样，成功冷冻保存和移植卵巢的其他测量值以及产卵数量的最高水平发生在供体和受体发育阶段的相同组合中。卵巢/受体发育阶段的其他组合产生较低的结果。第二次试验中，采集雄蛾精液，冷冻保存，解冻后的精液用胰蛋白酶溶液稀释，人工授精给在第一次试验最佳条件下获得的雌蛾。与对照组（100%）相比，受精蛾的产卵率很低（8-10.3%）。试验雌蛾卵的受精率低于对照雌蛾（分别为40.3 ~ 88%和97.5%）。在第三个实验中，在移植解冻的卵巢后，通过手术从蛾的卵巢小管中取出卵，并进行孤雌激活和人工孵化。正如预期的那样，所有产生的蛾都是雌性，在自然交配或用解冻的精液人工授精后，都能以很高的比率产生正常的后代。(C)2003 Elsevier Science（美国）。All rights reserved.
A reliable method is reported for the long-term preservation of ovaries and spermatozoa of the silkworm (Bombyx mori). Three studies are presented. In the first, ovaries were removed from larvae at either 3rd, 4th, or 5th instar, cryopreserved, and stored in liquid nitrogen. Thawed ovaries were transplanted to surgically castrated female larvae at the same or a different developmental stage. The highest percentage of recipient females producing eggs resulted into either 3rd or 4th instar larvae (respectively, 22.1 and 8.7%). Similarly, the highest levels of other measurements of successful cryopreservation and transplanted ovary, and number of eggs laid, occurred with the same combination of donor and recipient developmental stages. Other combinations of ovary/recipient developmental stages yielded lower results. In the second experiment, semen was collected from male moths, cryopreserved, and then thawed semen was diluted with trypsin solution and artificially inseminated into females obtained from the best conditions of first experiment. A small percentage of inseminated moths laid eggs (8-10.3%) compared to that of controls (100%). In addition, the fertility of eggs from experimental moths was lower than that of control females (respectively, 40.3-88% and 97.5%). In the third experiment, eggs were surgically removed from ovarian tubules of moth following transplantation of thawed ovaries and subjected to parthenogenetic activation and artificial hatching. As expected, all resulting moths were female and, following natural mating or artificial insemination with thawed semen, yielded normal offspring at high rates. (C) 2003 Elsevier Science (USA). All rights reserved.