Biogeography of soil archaea and bacteria along a steep precipitation gradient
Biogeography of soil archaea and bacteria along a steep precipitation gradient
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DOI：
10.1038/ismej.2009.136
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发表时间：
2010-04-01
期刊：
影响因子：
11
通讯作者：
Gillor, Osnat
中科院分区：
文献类型：
--
作者：
Angel, Roey;Soares, M. Ines M.;Gillor, Osnat
几个世纪以来，生物多样性一直困扰着生物学家，他们主要关注大型生物的多样性，而几乎忽视了微生物群落的地理动态。我们调查了土壤细菌和古菌的多样性，沿着一个陡峭的降水梯度，从以色列南部的内盖夫沙漠（900毫米的年降雨量）。土壤样品从三个长期生态研究站，从两种类型的补丁：植物间隙和下面的主要多年生植物在每个站点收集的三重样地。每个土壤样品的分子指纹采用16 S rRNA基因的末端限制性长度多态性，以评估细菌和古菌群落组成和多样性内和跨网站。群落组成的差异在统计学上不显着的网站内（P = 0.33和0.77的细菌和古菌，分别），但它不同的生态系统类型。这些差异在很大程度上可以解释结合植被覆盖的降水梯度：古菌和细菌的操作分类单位是独特的每个气候区域，即干旱，半干旱和地中海（P = 0.0001，两个域），以及补丁类型（P = 0.009和0.02，分别为细菌和古菌）。我们的研究结果表明，与地中海生态系统中的沙漠站点相比，更多样化的大型生物不同，古菌和细菌的diligence不受降水的限制。然而，群落的组成是独特的气候和植被覆盖，描绘每个生态系统。The ISME Journal（2010）4，553-563; doi：10.1038/ismej.2009.136; 2009年12月24日在线发表
For centuries, biodiversity has spellbound biologists focusing mainly on macroorganism's diversity and almost neglecting the geographic mediated dynamics of microbial communities. We surveyed the diversity of soil bacteria and archaea along a steep precipitation gradient ranging from the Negev Desert in the south of Israel (900mm annual rain). Soil samples were retrieved from triplicate plots at five long-term ecological research stations, collected from two types of patches: plant interspaces and underneath the predominant perennial at each site. The molecular fingerprint of each soil sample was taken using terminal restriction length polymorphism of the 16S rRNA gene to evaluate the bacterial and archaeal community composition and diversity within and across sites. The difference in community compositions was not statistically significant within sites (P = 0.33 and 0.77 for bacteria and archaea, respectively), but it differed profoundly by ecosystem type. These differences could largely be explained by the precipitation gradient combined with the vegetation cover: the archaeal and bacterial operational taxonomic units were unique to each climatic region, that is, arid, semiarid and Mediterranean (P = 0.0001, for both domains), as well as patch type (P = 0.009 and 0.02 for bacteria and archaea, respectively). Our results suggest that unlike macroorganisms that are more diverse in the Mediterranean ecosystems compared with the desert sites, archaeal and bacterial diversities are not constrained by precipitation. However, the community composition is unique to the climate and vegetation cover that delineates each ecosystem. The ISME Journal (2010) 4, 553-563; doi:10.1038/ismej.2009.136; published online 24 December 2009