CULTIVATION OF CELL-POLYMER CARTILAGE IMPLANTS IN BIOREACTORS
CULTIVATION OF CELL-POLYMER CARTILAGE IMPLANTS IN BIOREACTORS
复制标题
DOI：
10.1002/jcb.240510304
复制
发表时间：
1993-03-01
影响因子：
4
通讯作者：
LANGER, R
中科院分区：
文献类型：
--
作者：
FREED, LE;VUNJAKNOVAKOVIC, G;LANGER, R
可能用于重建或骨科手术的软骨植入物可以通过在合成的、可生物降解的聚合物支架上体外培养分离的软骨细胞（软骨细胞）来制造。支架提供了特定的三维结构，其支持细胞增殖和以受控的方式生物降解，与软骨组织的细胞再生平行。最近显示基于软骨细胞和纤维聚乙醇酸支架的软骨植入物在组织学上以及在细胞密度和基质组成（胶原、糖胺聚糖）方面与正常软骨非常相似[Freed等人，J Biomed Mater Res 27：11-23，1993a]。这些研究结果形成的基础上，开发简单的程序，以获得植入物用于临床使用的小，自体软骨标本没有任何限制，在可用性的供体组织或植入物dimensions.Chondrocyte生长和软骨基质再生的聚合物支架是相互依赖的，也取决于在体外组织培养条件。在静态培养条件下，由于软骨基质再生导致细胞质量增加和有效植入物孔隙率降低，细胞生长速率受到扩散限制。因此，体外培养环境的优化对于培养大型临床有用的软骨植入物至关重要。初步研究表明，主要的改进可以实现使用生物反应器，提供有效的传质和控制剪切速率在细胞和植入物表面。
Cartilage implants for potential use in reconstructive or orthopedic surgery can be created by growing isolated cartilage cells (chondrocytes) in vitro on synthetic, biodegradable polymer scaffolds. The scaffolds provide specific three-dimensional structures which support cell proliferation and biodegrade in a controlled fashion in parallel to cellular regeneration of cartilaginous tissue. Cartilage implants based on chondrocytes and fibrous polyglycolic acid scaffolds were recently shown to closely resemble normal cartilage histologically as well as with respect to cell density and matrix composition (collagen, glycosaminoglycan) [Freed et al., J Biomed Mater Res 27:11-23, 1993a]. These findings form the basis for developing straightforward procedures to obtain implants for clinical use from small, autologous cartilage specimens without any limitations in terms of availability of donor tissue or implant dimensions.Chondrocyte growth and cartilage matrix regeneration on polymer scaffolds are interdependent and also depend on in vitro tissue culture conditions. Under static culture conditions, cell growth rates are diffusionally limited due to increasing cell mass and decreasing effective implant porosity resulting from cartilage matrix regeneration. Optimization of the in vitro culture environment is thus essential for the cultivation of large, clinically useful cartilage implants. Preliminary studies indicate that major improvements can be achieved using bioreactors that provide efficient mass transfer and controlled shear rates at the cell and implant surfaces.