Phenotypical characterization of the rat striatal neurons expressing muscarinic receptor genes
Phenotypical characterization of the rat striatal neurons expressing muscarinic receptor genes
复制标题
表达毒蕈碱受体基因的大鼠纹状体神经元的表型特征
DOI：
10.1523/jneurosci.12-09-03591.1992
复制
发表时间：
1992
期刊：
影响因子：
64.8
通讯作者：
B. Bloch
中科院分区：
文献类型：
--
作者：
V. Bernard;E. Normand;B. Bloch
通过使用多种原位杂交和免疫组织化学方法，鉴定并表征了成年大鼠纹状体中表达 m1、m2 和 m4 毒蕈碱受体基因的神经元。结合原位杂交同时检测同一切片或相邻切片中的两个 mRNA，以及对相邻切片进行原位杂交和免疫组织化学，使我们能够鉴定含有 m1、m2 或 m4 受体 mRNA 的神经元。我们的观察表明，m1、m2 和 m4 受体基因在一个或多个表型不同的神经元群体中表达。 m1 受体基因表达最广泛（85% 的纹状体神经元）。大多数胆碱能神经元（80% 或更多）含有 m1、m2 和 m4 受体 mRNA。几乎所有P物质神经元都含有m1和m4受体mRNA。所有脑啡肽能神经元都含有 m1 受体 mRNA，但只有 39% 含有 m4 受体 mRNA。大多数生长抑素和神经降压素神经元表达 m1 受体基因，但只有少数（分别为 15% 和 9%）含有 m4 受体 mRNA。本研究提供的解剖学证据表明，ACh 可能通过毒蕈碱受体以复杂的方式直接作用于纹状体的主要神经元群。 m1、m2 和 m4 受体可能充当自身受体来控制 ACh 释放以及可能的 ACh 神经元的其他参数。另一方面，m1 和 m4 受体可能充当胆碱感受传出神经元（脑啡肽和 P 物质神经元）和其他神经元（生长抑素/神经肽 Y 和神经降压素神经元）中的异质受体。仅部分脑啡肽、生长抑素和神经降压素神经元群中存在 m4 受体 mRNA，表明毒蕈碱受体基因表达有助于纹状体的功能和解剖异质性，这可能与更高级别的组织相关，包括斑块基质区隔化。 m1 和 m4 受体基因在纹状体中的广泛表达表明 ACh 可能直接影响纹状体传出神经元和内在神经元中神经递质的释放和合成。我们的结果表明，毒蕈碱受体基因的特定表达模式介导ACh对化学和拓扑定义的纹状体神经元群的活动和功能的直接影响。由于毒蕈碱受体的表达发生在纹状体的三个主要神经元群中，即乙酰胆碱、脑啡肽和也表达多巴胺受体的P物质神经元，因此乙酰胆碱和多巴胺很可能在单细胞水平上共同作用，影响纹状体功能。
Neurons expressing the m1, m2, and m4 muscarinic receptor genes in the adult rat striatum were identified and characterized by using several in situ hybridization and immunohistochemical procedures. Combined in situ hybridization for the simultaneous detection of two mRNAs in the same section or in adjacent sections as well as in situ hybridization and immunohistochemistry on adjacent sections permitted us to identify the neurons containing m1, m2, or m4 receptor mRNA. Our observations demonstrate that m1, m2, and m4 receptor genes are expressed in one or several phenotypically distinct neuronal populations. The m1 receptor gene was the most widely expressed (85% of the striatal neurons). Most cholinergic neurons (80% or more) contain m1, m2, and m4 receptor mRNAs. Almost all the substance P neurons contain m1 and m4 receptor mRNA. All enkephalinergic neurons contained m1 receptor mRNA, but only 39% contained m4 receptor mRNA. Most somatostatin and neurotensin neurons expressed the m1 receptor gene, but only a few (15% and 9%, respectively) contained m4 receptor mRNA. The present study offers anatomical evidence that ACh may act directly in complex ways on the main neuronal populations of the striatum through muscarinic receptors. The m1, m2, and m4 receptors may act as autoreceptors to control ACh release and possibly other parameters of ACh neurons. On the other hand, the m1 and m4 receptors may act as heteroreceptors in cholinoceptive efferent neurons (enkephalin and substance P neurons) and other neurons (somatostatin/neuropeptide Y and neurotensin neurons). The presence of m4 receptor mRNA in only parts of the enkephalin, somatostatin, and neurotensin neuronal populations indicates that muscarinic receptor gene expression contributes to the functional and anatomical heterogeneity of the striatum that may relate to higher order of organization, including patch-matrix compartmentalization. The wide expression of m1 and m4 receptor genes in the striatum suggests that ACh may directly influence neurotransmitter release and synthesis in striatal efferent and intrinsic neurons. Our results imply that the specific pattern of expression of the muscarinic receptor genes mediates direct effects of ACh on activities and functions of chemically and topologically defined striatal neuronal populations. Since the expression of muscarinic receptors occurred in the three main neuronal populations of the striatum, namely ACh, enkephalins, and substance P neurons that also express dopamine receptors, it is highly probable that ACh and dopamine may act together at the single-cell level to influence striatal functions.
毒蕈碱受体阻断增加纹状体切片的基础乙酰胆碱释放。
DOI：
--
发表时间：
1990
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Dolezal,V;Wecker,L
通讯作者：
Wecker,L
DOI：
10.1016/s0021-9258(18)68881-7
发表时间：
1988-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Kislauskis;B. Bullock;S. McNeil;P. Dobner
通讯作者：
E. Kislauskis;B. Bullock;S. McNeil;P. Dobner