The use of minimum selectable concentrations (MSCs) for determining the selection of antimicrobial resistant bacteria.
The use of minimum selectable concentrations (MSCs) for determining the selection of antimicrobial resistant bacteria.
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DOI：
10.1007/s10646-017-1762-y
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Knapp CW
中科院分区：
文献类型：
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作者：
Khan S;Beattie TK;Knapp CW
在许多工业中，特别是饮用水生产中，使用抗菌化合物来根除微生物是必不可少的。然而，细菌生长在消毒剂浓度通常是致命的情况下并不罕见，因为细菌种群可以产生耐药性。群体耐药性的常用指标是基于最低抑制浓度（MIC），这是基于细菌的致死率。然而，由于细菌生长速度的差异，亚致死浓度也可能选择耐药细菌。本研究确定了暴露于游离氯和单氯胺的细菌种群的最小选择浓度（MSCs），这一指标可能比MIC更好地反映了在较低消毒剂水平下发生的选择压力。对系统发育相似的细菌进行一系列浓度的消毒剂挑战。游离氯和单氯胺的MSCs浓度在0.021 ~ 0.39 mg L−1之间，是敏感菌MICsusc浓度的1/250 ~ 1/5。本研究表明，亚致死浓度的消毒剂可能导致耐药菌群的选择，而间充质干细胞将是一个更敏感的选择压力指标，特别是在环境系统中。
The use of antimicrobial compounds is indispensable in many industries, especially drinking water production, to eradicate microorganisms. However, bacterial growth is not unusual in the presence of disinfectant concentrations that would be typically lethal, as bacterial populations can develop resistance. The common metric of population resistance has been based on the Minimum Inhibitory Concentration (MIC), which is based on bacteria lethality. However, sub-lethal concentrations may also select for resistant bacteria due to the differences in bacterial growth rates. This study determined the Minimal Selective Concentrations (MSCs) of bacterial populations exposed to free chlorine and monochloramine, representing a metric that possibly better reflects the selective pressures occurring at lower disinfectant levels than MIC. Pairs of phylogenetically similar bacteria were challenged to a range of concentrations of disinfectants. The MSCs of free chlorine and monochloramine were found to range between 0.021 and 0.39 mg L−1, which were concentrations 1/250 to 1/5 than the MICs of susceptible bacteria (MICsusc). This study indicates that sub-lethal concentrations of disinfectants could result in the selection of resistant bacterial populations, and MSCs would be a more sensitive indicator of selective pressure, especially in environmental systems.