Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana
Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana
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DOI：
10.1007/s00438-007-0315-0
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发表时间：
2008-03-01
影响因子：
3.1
通讯作者：
Ichinose, Yuki
中科院分区：
文献类型：
--
作者：
Higashi, Kuniaki;Ishiga, Yasuhiro;Ichinose, Yuki
鞭毛蛋白是细菌假单胞菌致病变种（Pseudomonasseringae pv.）鞭毛丝的一种成分。tabaci 6605（Pta）在非寄主拟南芥中诱导过敏反应。WRKY41基因属于一个多基因家族，编码WRKY植物特异性转录因子。thaliana. WRKY41的表达通过接种不相容的病原体P.番茄DC3000（Pto）接种AvrRpt 2和非寄主病原菌Pta后6h内，接种亲和性Pto后，则不能分离出AvrRpt 2和Pta。WRKY41的表达也通过接种A.拟南芥与Pto的hrp型三分泌系统（T3SS）缺陷型突变体，表明Pto野生型中由T3SS产生的效应子抑制WRKY41的活化。过表达WRKY41的拟南芥表现出对Pto野生型的抗性增强，但对胡萝卜软腐欧文氏菌EC1的易感性增加。WRKY41过表达拟南芥组成型表达PR5基因，但抑制茉莉酸甲酯诱导的PDF1.2基因表达。这些结果表明，WRKY41可能是水杨酸和茉莉酸途径的串扰的关键调节剂。
Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.