Calculating order parameter profiles utilizing magnetically aligned phospholipid bilayers for 2H solid-state NMR studies
Calculating order parameter profiles utilizing magnetically aligned phospholipid bilayers for 2H solid-state NMR studies
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DOI：
10.1016/s0926-2040(03)00052-3
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发表时间：
2003-09-01
影响因子：
3.2
通讯作者：
Lorigan, GA
中科院分区：
文献类型：
--
作者：
Dave, PC;Tiburu, EK;Lorigan, GA
利用固体氘核磁共振谱研究了硬脂酸-d（35）在磁性排列磷脂双层膜中的结构和动力学性质随温度的变化。磁性排列的磷脂双层或双胞是模型系统，其模拟生物膜用于磁共振研究。添加顺磁性镧系元素离子（Yb 3+）以对齐双胞，使得双层法线与静磁场的方向共线。计算了硬脂酸-d（35）探针的相应有序参数，并与文献中未取向样品的值进行了比较。胆固醇加入到bicelle系统中会降低磷脂双层的流动性，并增加硬脂酸-d酰基链的有序性（35）。这项研究表明，利用磁性排列的bicelles计算H-2序参数配置文件的非生物系统，如聚合物接枝膜和希夫碱配合物的可行性。(C)2003年爱思唯尔公司All rights reserved.
Solid-state deuterium NMR spectroscopy was used to study the structural and dynamic properties of stearic acid-d(35) in magnetically aligned phospholipid bilayers as a function of temperature. Magnetically aligned phospholipid bilayers or bicelles are model systems, which mimic biological membranes for magnetic resonance studies. Paramagnetic lanthanide ions (Yb3+) were added to align the bicelles such that the bilayer normal is colinear with the direction of the static magnetic field. The corresponding order parameters of the stearic acid-d(35) probe were calculated and compared with values obtained from unoriented samples in the literature. The addition of cholesterol to the bicelle system decreases the fluidity of the phospholipid bilayers and increases the ordering of the acyl chains of stearic acid-d(35). This study demonstrates the feasibility of utilizing magnetically aligned bicelles for calculating H-2 order parameter profiles for non-biological systems such as polymer-grafted membranes and Schiff's base complexes. (C) 2003 Elsevier Inc. All rights reserved.