Unbiased phosphoproteome profiling uncovers novel phosphoproteins and phosphorylation motifs in bermudagrass stolons
Unbiased phosphoproteome profiling uncovers novel phosphoproteins and phosphorylation motifs in bermudagrass stolons
复制标题
无偏磷酸蛋白质组分析揭示了狗牙根匍匐茎中的新型磷蛋白和磷酸化基序
DOI：
10.1016/j.plaphy.2019.09.036
复制
发表时间：
2019
影响因子：
6.5
通讯作者：
Jianxiu Liu
中科院分区：
文献类型：
--
作者：
Bing Zhang;Jingbo Chen;Junqin Zong;Xuebing Yan;Jianxiu Liu
As a widely used turfgrass species, bermudagrass (Cynodon dactylonL.) can be easily propagated through colonial growth of stolons. Previous studies collectively revealed that exotic environmental factors and intrinsic hormones and genes are all involved in the differentiation, development, and diageotropical growth of stolons. However, the detailed molecular mechanism how environmental and hormone signals regulate the gene expression and biochemical activities in bermudagrass stolons remains unclear. In this study, we observed that reversible phosphorylation modification plays important roles in normal growth and physiological functions of bermudagrass stolons. LC-MS/MS analyses of the total protein extracts of bermudagrass stolons without preliminary phosphopeptide-enrichment successfully identified 646 nonredundant phosphorylation sites and 485 phosphoproteins. The phosphoproteins were significantly enriched in protein phosphorylation regulation and starch metabolism processes. Motif-X analyses further revealed that phosphoproteins containing novel phosphorylation motifs might be involved in transcription regulation of bermudagrass stolons. These results greatly expanded our understanding of the growth and development of bermudagrass stolons at the post-translational level.
