Identification of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry
Identification of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry
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DOI：
10.1021/ac020109k
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发表时间：
2002-11-01
影响因子：
7.4
通讯作者：
Wang, J
中科院分区：
文献类型：
--
作者：
Du, ZM;Yang, RF;Wang, J
为评价基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）在鉴定金黄色葡萄球菌及其耐甲氧西林性方面的性能，我们分析了76株金黄色葡萄球菌。使用线性MALDI-TOF MS分析金黄色葡萄球菌临床分离株。将获得的光谱分布数据与仪器提供的数据库进行比较，74%的分离株被鉴定为S.金黄色葡萄球菌，如通过基于核酸的PCR测试所证实的。金黄色葡萄球菌甲氧西林耐药性的测定是基于甲氧西林敏感的金黄色葡萄球菌的光谱特征。金黄色葡萄球菌（MSSA）和耐甲氧西林的S.金黄色葡萄球菌（MRSA）彼此之间差异很大。从每个分离物获得的重复光谱轮廓被组合为它的代表性光谱，并且来自所有分离物的代表性光谱轮廓构成用户自建立的数据库。根据聚类分析结果，将数据库中的所有谱图分为两组，一组是MSSA，另一组是MRSA。MALDI-TOF MS与VCR的结果有一定差异。7株mecA基因PCR阴性的分离株经MALDI-TOF MS鉴定为MRSA。金黄色。结果表明，微生物的MALDI-TOF MS谱图比较可以作为细菌鉴定和抗生素敏感性分析的一种简单、快速的方法。
To evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying Staphylococcus aureus and in determining its methicillin resistance, we analyzed 76 S. aureus clinical isolates using a linear MALDI-TOF MS. Spectral profile data obtained were compared with the database provided with the instrument, and 74% of the isolates were identified as S. aureus, as confirmed by a nuc-based PCR test. The determination of the methicillin resistance in S:,aureus is based on the fact that the spectral profiles of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) differ greatly from each other. Replicate spectral profiles obtained from each isolate were combined to be a representative spectrum of it, and representative spectral profiles from all the isolates constitute a user's self established database. All the spectral profiles in the database were classified into two groups based on clustering analysis, and one is for MSSA and another MRSA. There was a little discrepancy between the results from MALDI-TOF MS and from VCR. Seven isolates that are negative for the mecA gene by PCR were identified as MRSA by MALDI-TOF MS. The discrepancy may be partially explained by the heterogeneous nature of methicillin resistance in S. aureus. Our results suggested that comparison of MALDI-TOF MS spectral profiles of microorganism could serve as a simple and rapid method for bacterial identification and antibiotic susceptibility analysis.