Comparison of three decontamination methods for Mycobacterium bovis isolation.
Comparison of three decontamination methods for Mycobacterium bovis isolation.
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DOI：
10.1590/s1517-83822008000200008
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发表时间：
2008-04
期刊：
影响因子：
--
通讯作者：
Amaku M
中科院分区：
文献类型：
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作者：
Ambrosio SR;de Deus Oliveira EM;Rodriguez CA;Ferreira Neto JS;Amaku M
Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
