Osteocyte dendrogenesis in static and dynamic bone formation: An ultrastructural study
Osteocyte dendrogenesis in static and dynamic bone formation: An ultrastructural study
复制标题
DOI：
10.1002/ar.a.20032
复制
发表时间：
2004-05-01
期刊：
影响因子：
--
通讯作者：
Marotti, G
中科院分区：
文献类型：
--
作者：
Palumbo, C;Ferretti, M;Marotti, G
目前对骨细胞树突发生的超微结构研究代表了先前研究的延续（Ferretti等人，解剖胚胎，二○ ○二年; 206：21-29），其中我们指出，在膜内骨化期间，由迁移的成骨细胞板层进行的众所周知的动态骨形成（DBF）之前是静态骨形成（SBF），其中静止成骨细胞的索在它们分化的相同部位转化为骨细胞。本文对鸡胚和新生兔各种长骨中段周围的软骨膜骨化中心进行了研究。透射电镜观察表明，两种成骨方式中骨细胞树突的形成有很大差异，主要取决于成骨细胞运动是否发生。在DBF中，成骨细胞转化为小的卵圆形/椭圆形骨细胞，并且它们的树突以异步和不对称的方式形成，伴随着并依赖于前进的矿化表面和后退的成骨层。在SBF中，静止的成骨细胞产生大的球形骨细胞，位于融合的陷窝内，具有短而对称的树突，这些树突可以同时围绕其细胞体辐射，因为它们完全被未矿化基质包围。在所有骨细胞（SBF衍生和DBF衍生）之间以及骨细胞和成骨细胞之间观察到接触和间隙连接。最后，一个连续的骨细胞网络延伸到整个骨骼，无论其静态或动态的起源。该网络具有功能性合胞体的特征，其潜在地能够通过布线传输来调节覆盖骨表面的成骨谱系的细胞。(C)2004 Wiley-Liss，Inc.
The present ultrastructural investigation into osteocyte dendrogenesis represents a continuation of a previous study (Ferretti et al., Anat. Embryol., 2002; 206:21-29), in which we pointed out that, during intramembranous ossification, the well-known dynamic bone formation (DBF), performed by migrating osteoblast laminae, is preceded by static bone formation (SBF), in which cords of stationary osteoblasts transform into osteocytes in the same site where they differentiated. The research was carried out on the perichondral center of ossification surrounding the mid shaft level of various long bones of chick embryos and newborn rabbits. Transmission electron microscope observations showed that the formation of osteocyte dendrites is quite different in the two types of osteogenesis, mainly depending on whether or not osteoblast movement occurs. In DBF, osteoblasts transform into small ovoidal/ellipsoidal osteocytes and their dentrites form in an asynchronous and asymmetrical manner in concomitance with, and depending on, the advancing mineralizing surface and the receding osteogenic laminae. In SBF, stationary osteoblasts give rise to big globous osteocytes, located inside confluent lacunae, with short and symmetrical dendrites that can radiate simultaneously all around their cell body because they are completely surrounded by unmineralized matrix. Contacts and gap junctions were observed between all osteocytes (both SBF- and DBF-derived) and between osteocytes and osteoblast's. Finally, a continuous osteocyte network extends throughout the bone, regardless of its static or dynamic origin. This network has the characteristic of a functional syncytium, potentially capable of modulating, by wiring transmission, the cells of the osteogenic lineage covering the bone surfaces. (C) 2004 Wiley-Liss, Inc.