Nonomuraea aurantiaca sp. nov., a novel cellulase-producing actinobacterium isolated from soil.
Nonomuraea aurantiaca sp. nov., a novel cellulase-producing actinobacterium isolated from soil.
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DOI：
10.1099/ijsem.0.005411
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发表时间：
2022-06
影响因子：
2.8
通讯作者：
Jiaying Lin;Li Zhang;Lulu Qian;Yanming Yang;W. Xiang;Junwei Zhao;Xiangjing Wang
中科院分区：
文献类型：
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作者：
Jiaying Lin;Li Zhang;Lulu Qian;Yanming Yang;W. Xiang;Junwei Zhao;Xiangjing Wang
从黑龙江省齐齐哈尔市土壤中分离到一株产纤维素酶放线菌，命名为NEAU-L178 T。进行了多相研究，以确定菌株的分类地位。根据16 S rRNA基因序列分析，菌株NEAU-L178 T应归属于野野村菌属，与海绵野野村菌SYSU K10005 T亲缘关系较近（99.31%16S rRNA基因序列相似性），大豆野野村菌NEAU-BB 2C 19 T（98.75%）、广州野野村菌NEAU-ZJ 3 T（98.75%）和根球野野村菌NEAU-mq 18 T（98.34%）。它们之间的数字DNA-DNA杂交值分别为27.1、26.1、42.0和30.9%，它们之间的全基因组平均核苷酸同一性值分别为83.1、82.3、90.3和85.8%。全细胞水解产物含有葡萄糖、核糖、阿拉伯糖和马杜糖。甲基萘醌经鉴定为MK-9（H 0）、MK-9（H4）和MK-9（H2）。主要脂肪酸为C16：0、iso-C17：0和C17：0 10-甲基。检测到的极性脂质为二磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰肌醇和三种未鉴定的磷脂。基因组DNA G+C含量为69.7mol%。此外，全基因组分析表明，菌株NEAU-L178 T具有降解纤维素的潜力。根据表型、基因型、化学分类学和系统发育学数据，菌株NEAU-L178 T可以与其近缘系统发育相关物区分开来，并代表了野野村菌属的一个新种，建议将其命名为橙色野野村菌（Nonomuraea aurantiaca sp. nov.）。模式菌株为NEAU-L178 T（=JCM 34799 T = CGMCC4.7741T）。
A novel cellulase-producing actinobacterium, designated strain NEAU-L178T, was isolated from soil sample collected from Qiqihaer, Heilongjiang Province, PR China. A polyphasic study was carried out to determine the taxonomic status of the strain. On the basis of 16S rRNA gene sequence analysis, strain NEAU-L178T should be classified into the genus Nonomuraea and is closely related to Nonomuraea cavernae SYSU K10005T (99.31 % 16S rRNA gene sequence similarity), Nonomuraea glycinis NEAU-BB2C19T (98.75 %), Nonomuraea guangzhouensis NEAU-ZJ3T (98.75 %) and 'Nonomuraea rhizosphaerae' NEAU-mq18T (98.34 %). The digital DNA-DNA hybridization values between them are 27.1, 26.1, 42.0 and 30.9 %, and the whole-genome average nucleotide identity values between them are 83.1, 82.3, 90.3 and 85.8 %, respectively. The whole-cell hydrolysates contained glucose, ribose, arabinose and madurose. The menaquinones were identified as MK-9(H0), MK-9(H4) and MK-9(H2). The major fatty acids were C16 : 0, iso-C17 : 0 and C17 : 0 10-methyl. The detected polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and three unidentified phospholipids. The genomic DNA G+C content was 69.7 mol%. In addition, whole-genome analysis indicated that strain NEAU-L178T had the potential to degrade cellulose. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic data, strain NEAU-L178T can be differentiated from its close phylogenetic relatives and represents a novel species of the genus Nonomuraea, for which the name Nonomuraea aurantiaca sp. nov. is proposed. The type strain is NEAU-L178T (=JCM 34799T=CGMCC 4.7741T).