Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast
Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast
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DOI：
10.1073/pnas.152264899
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发表时间：
2002-08-06
影响因子：
11.1
通讯作者：
Romesberg, FE
中科院分区：
文献类型：
--
作者：
Hanway, D;Chin, JK;Romesberg, FE
竞争性生长试验已被用来确定酵母基因参与修复紫外线或MMS诱导的DNA损伤。分析了2，827个酵母菌株的集合，其中每个菌株具有被包含两个独特序列标签的盒替换的单个ORF，允许通过与高密度寡核苷酸阵列杂交来检测。杂交数据鉴定了高百分比的缺失菌株，其存在于先前被表征为对DNA损伤剂敏感的集合中。该试验和随后的分析，已被用来确定六个基因，以前不知道是参与损伤反应，其删除使酵母敏感的UV或MMS处理。最近鉴定的基因包括三个未表征的ORF，以及编码参与泛素化、基因沉默和跨线粒体膜转运的蛋白质产物的基因。对其中四个基因进行上位性分析，以确定蛋白质产物发挥功能的DNA损伤修复途径。
A competitive growth assay has been used to identify yeast genes involved in the repair of UV- or MMS-induced DNA damage. A collection of 2,827 yeast strains was analyzed in which each strain has a single ORF replaced with a cassette containing two unique sequence tags, allowing for its detection by hybridization to a high-density oligonucleotide array. The hybridization data identify a high percentage of the deletion strains present in the collection that were previously characterized as being sensitive to the DNA-damaging agents. The assay, and subsequent analysis, has been used to identify six genes not formerly known to be involved in the damage response, whose deletion renders the yeast sensitive to UV or MMS treatment. The recently identified genes include three uncharacterized ORFs, as well as genes that encode protein products implicated in ubiquitination, gene silencing, and transport across the mitochondrial membrane. Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function.